纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | C20orf81 |
Uniprot No | Q9H1Q7 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-454aa |
氨基酸序列 | MVFCLSSEEPRRPLRSDMVHFQASEVQQLLHNKFVVILGDSIQRAVYKDLVLLLQKDSLLTAAQLKAKGELSFEQDQLVAGGQLGELHNGTQYREVRQFCSGSGHHLVRFYFLTRVYSEYLEDVLEELTYGPAPDLVIINSCLWDLSRYGRCSMESYRENLERVFVRMDQVLPDSCLLVWNMAMPLGERITGGFLLPELQPLAGSLRRDVVEGNFYSATLAGDHCFDVLDLHFHFRHAVQHRHRDGVHWDQHAHRHLSHLLLTHVADAWGVELPKRGYPPDPWIEDWAEMNHPFQGSHRQTPDFGEHLALLPPPPSSLPPPMPFPYPLPQPSPPPLFPPLPQDTPFFPGQPFPPHEFFNYNPVEDFSMPPHLGCGPGVNFVPGPLPPPIPGPNPHGQHWGPVVHRGMPRYVPNSPYHVRRMGGPCRQRLRHSERLIHTYKLDRRPPAHSGTWPG |
分子量 | 75.68 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | 0 |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是几篇关于重组人C20orf81蛋白的参考文献示例(注:部分文献信息为假设性示例,实际研究中可能使用不同命名或内容):
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1. **文献名称**: *"Characterization of recombinant human C20orf81 as a novel regulator of autophagosome maturation"*
**作者**: Yamaguchi T, et al.
**摘要**: 本研究首次报道了C20orf81的重组蛋白表达及功能分析。通过体外实验发现,C20orf81通过与LC3相互作用促进自噬体-溶酶体融合,可能参与自噬调控通路,为神经退行性疾病研究提供了新靶点。
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2. **文献名称**: *"Structural insights into the oligomeric state of C20orf81 through cryo-EM analysis"*
**作者**: Li J, Smith RK.
**摘要**: 利用冷冻电镜解析了重组C20orf81蛋白的高分辨率结构,揭示其形成同源二聚体的能力及潜在的功能结构域,表明其可能作为支架蛋白参与细胞信号转导。
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3. **文献名称**: *"C20orf81 expression correlates with tumor progression and modulates EMT in colorectal cancer cells"*
**作者**: Wang L, et al.
**摘要**: 通过重组C20orf81蛋白的功能研究表明,其在结直肠癌细胞中过表达可激活上皮-间质转化(EMT),促进细胞迁移,提示其作为癌症治疗潜在生物标志物的可能性。
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4. **文献名称**: *"Recombinant C20orf81 exhibits ATPase activity and interacts with mitochondrial membrane proteins"*
**作者**: Chen H, et al.
**摘要**: 本研究在大肠杆菌中成功表达并纯化了重组C20orf81蛋白,发现其具有ATP水解酶活性,并通过质谱分析鉴定出与线粒体膜蛋白的相互作用,提示其在线粒体功能中的潜在作用。
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**备注**:以上文献为示例性内容,实际文献需通过数据库(如PubMed、Web of Science)以“C20orf81”或更新后的基因名称检索。若该蛋白研究较少,可尝试扩展至其功能相关的通路或疾病领域。
**Background of Recombinant Human C20orf81 Protein**
The C20orf81 (Chromosome 20 Open Reading Frame 81) gene encodes a protein that remains poorly characterized, with limited functional data available in public databases. It is evolutionarily conserved across mammals, suggesting potential biological significance. Structural predictions indicate that C20orf81 may contain transmembrane domains or signal peptides, hinting at roles in subcellular trafficking or membrane-associated processes. However, its exact molecular function, interacting partners, and physiological relevance are still under investigation.
Recombinant human C20orf81 protein is artificially expressed in model systems (e.g., *E. coli*, mammalian cells) to study its biochemical properties and cellular localization. Preliminary studies associate it with possible involvement in neurological or developmental pathways, though evidence remains speculative. Some bioinformatic analyses link C20orf81 to metabolic or immune regulation, but experimental validation is scarce.
Interest in C20orf81 has grown due to its differential expression in certain cancers and autoimmune disorders, implying potential biomarker or therapeutic applications. Current research focuses on elucidating its role in disease contexts and clarifying its mechanism of action using recombinant protein tools. Despite its enigmatic status, C20orf81 represents an intriguing target for unraveling novel biological pathways.
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