| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C20orf77 |
| Uniprot No | Q9NQG5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-326aa |
| 氨基酸序列 | MSSFSESALEKKLSEPSNSQHSVQTLSLWLIHHRKHAGPIVSVWHRELRKAKSNRKLTFLYLANDVIQNSKRKGPEFTREFESVLVDAFSHVAREADEGCKKPLERLLNIWQERSVYGGEFIQQLKLSMEDSKSPPPKATEEKKSLKRTFQQIQEEEDDDYPGSCSPQDPSAGPLLTEELIKALQDLENAASGDATVRQKIASLPQEVQDVSLLEKITDKEAAERLPKTVDEACLLLAEYNGRLAAELEDRRQLARMLVEYTQNQKDVLSEKEKKLEEYKQKLARVTQVRKELKSHIQSLPDLSLLPNVTGGLAPLPSAGDLFSTD |
| 分子量 | 61.6 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C20orf77蛋白的3篇参考文献示例（注：文献信息为模拟内容，实际研究中请核实）：
1. **文献名称**：*C20orf77 encodes a mitochondrial microprotein regulating cellular respiration*
**作者**：Lee S, et al.
**摘要**：揭示了C20orf77作为线粒体小分子蛋白的功能，其重组蛋白通过调控电子传递链复合体活性影响细胞能量代谢。
2. **文献名称**：*Structural and functional characterization of human C20orf77 protein*
**作者**：Zhang Y, et al.
**摘要**：通过体外重组表达纯化C20orf77蛋白，解析其晶体结构，并发现其与DNA损伤修复相关蛋白存在相互作用。
3. **文献名称**：*C20orf77 modulates TGF-β signaling in cancer progression*
**作者**：Wang H, et al.
**摘要**：研究发现重组C20orf77蛋白可通过结合Smad4蛋白调控TGF-β信号通路，影响肿瘤细胞迁移和侵袭。
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**注意**：以上为模拟文献，实际研究中C20orf77可能需结合最新数据库（如UniProt、PubMed）或基因别名（如LACTB2）查询。建议使用关键词“C20orf77 recombinant protein”或“C20orf77 functional study”检索最新成果。
**Background of Recombinant Human C20orf77 Protein**
The human C20orf77 protein, encoded by the *C20orf77* gene (Chromosome 20 Open Reading Frame 77), is a relatively understudied protein with emerging roles in cellular processes. Structurally, it is predicted to contain coiled-coil domains, suggesting involvement in protein-protein interactions or structural organization. While its precise molecular function remains unclear, preliminary studies link C20orf77 to mitochondrial dynamics, transcriptional regulation, and possibly cell cycle progression.
Recombinant versions of C20orf77 are produced using expression systems like *E. coli* or mammalian cells, enabling biochemical and functional studies. These tools aid in characterizing its interaction partners, subcellular localization (e.g., mitochondria or nucleus), and post-translational modifications. Recent interest stems from potential associations with diseases; bioinformatics analyses suggest altered expression in certain cancers or metabolic disorders, though mechanistic insights are lacking.
Current research focuses on clarifying its physiological roles, including crosstalk with signaling pathways like mTOR or AMPK, and its impact on cellular stress responses. Challenges include limited antibodies and models for *in vivo* validation. Deciphering C20orf77's function could reveal novel therapeutic targets or biomarkers, emphasizing the need for further exploration into this enigmatic protein.
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