| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C20orf65 |
| Uniprot No | Q9H1H1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-148aa |
| 氨基酸序列 | MEPEAFEICPYDPHHRIPLSRFQYHLASCRRKNPKKAKKMATCKYNACHVVPIKNLEEHEAVCVNRSAVEEEDTENPLKVSPPSSEQNDDTQQVSPCLPSPDIWNVDGANCQHVFVLKTFFPQKVVCENDTKESARETSPQKILRPGQ |
| 分子量 | 43.3 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C20orf65蛋白的模拟参考文献示例（仅供示例，具体文献需根据实际研究补充）：
1. **标题**：*A novel C20orf65 mutation causes male infertility with teratozoospermia via disrupting protein stability*
**作者**：Li, Y., et al. (2021)
**摘要**：研究报道C20orf65基因突变与男性不育相关，通过重组表达突变体蛋白，发现突变导致蛋白结构不稳定，影响其在精子形成中的功能。
2. **标题**：*C20orf65 is a cilia-associated protein essential for respiratory cilia motility*
**作者**：Smith, J., et al. (2020)
**摘要**：利用重组C20orf65蛋白进行体外实验，发现其与纤毛轴丝组分相互作用，敲除后导致纤毛运动缺陷，提示其在呼吸道疾病中的作用。
3. **标题**：*Characterization of C20orf65 function in mitochondrial metabolism using recombinant expression models*
**作者**：Zhang, R., et al. (2019)
**摘要**：通过大肠杆菌重组表达C20orf65蛋白，分析其酶活性和亚细胞定位，揭示其在线粒体能量代谢中的潜在作用。
4. **标题**：*CRISPR/Cas9-mediated knockout and overexpression of C20orf65 in cell lines reveals roles in DNA repair*
**作者**：Garcia, M., et al. (2022)
**摘要**：构建重组C20orf65过表达细胞系，结合功能实验证明其参与调控DNA损伤修复通路。
（注：以上文献为示例，实际引用需以真实发表文献为准。）
**Background of Recombinant Human C20orf65 Protein**
The C20orf65 (Chromosome 20 Open Reading Frame 65) gene encodes a protein predominantly expressed in tissues with high ciliary activity, such as the testes and respiratory epithelium. While its precise molecular function remains unclear, C20orf65 is implicated in cilia-related processes, potentially influencing motility or signaling. Studies link C20orf65 mutations to male infertility due to sperm flagellar defects and primary ciliary dyskinesia (PCD), a disorder characterized by dysfunctional motile cilia leading to chronic respiratory infections and laterality defects.
Recombinant human C20orf65 protein is engineered via heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its structure, interactions, and role in ciliopathies. Its production enables functional analyses, antibody development, and exploration of disease mechanisms. Recent investigations suggest C20orf65 may localize to the axoneme of cilia and flagella, interacting with proteins involved in ciliary assembly or motility. However, its exact biochemical pathways and regulatory mechanisms remain under investigation.
Research on recombinant C20orf65 holds therapeutic potential for ciliary disorders and infertility. Challenges include clarifying its functional domains and validating disease-associated variants. Advances in structural biology and model organisms may accelerate understanding of this enigmatic protein's contribution to cellular and pathological processes.
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