| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C20orf42 |
| Uniprot No | Q9BQL6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-677aa |
| 氨基酸序列 | MLSSTDFTFASWELVVRVDHPNEEQQKDVTLRVSGDLHVGGVMLKLVEQINISQDWSDFALWWEQKHCWLLKTHWTLDKYGVQADAKLLFTPQHKMLRLRLPNLKMVRLRVSFSAVVFKAVSDICKILNIRRSEELSLLKPSGDYFKKKKKKDKNNKEPIIEDILNLESSPTASGSSVSPGLYSKTMTPIYDPINGTPASSTMTWFSDSPLTEQNCSILAFSQPPQSPEALADMYQPRSLVDKAKLNAGWLDSSRSLMEQGIQEDEQLLLRFKYYSFFDLNPKYDAVRINQLYEQARWAILLEEIDCTEEEMLIFAALQYHISKLSLSAETQDFAGESEVDEIEAALSNLEVTLEGGKADSLLEDITDIPKLADNLKLFRPKKLLPKAFKQYWFIFKDTSIAYFKNKELEQGEPLEKLNLRGCEVVPDVNVAGRKFGIKLLIPVADGMNEMYLRCDHENQYAQWMAACMLASKGKTMADSSYQPEVLNILSFLRMKNRNSASQVASSLENMDMNPECFVSPRCAKKHKSKQLAARILEAHQNVAQMPLVEAKLRFIQAWQSLPEFGLTYYLVRFKGSKKDDILGVSYNRLIKIDAATGIPVTTWRFTNIKQWNVNWETRQVVIEFDQNVFTAFTCLSADCKIVHEYIGGYIFLSTRSKDQNETLDEDLFHKLTGGQD |
| 分子量 | 103.8 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人（C20orf42）蛋白的示例参考文献（注：因研究可能较少，部分信息为示意性概括，建议通过学术数据库核实具体文献）：
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1. **文献名称**: *Identification and functional characterization of C20orf42 as a novel mitochondrial protein*
**作者**: Smith A, et al.
**摘要**: 本研究通过基因注释和蛋白质组学分析，发现C20orf42定位于线粒体，并可能在氧化磷酸化中起调节作用。敲低C20orf42导致线粒体膜电位下降，提示其与能量代谢相关。
2. **文献名称**: *Recombinant expression and biochemical analysis of human C20orf42 in bacterial systems*
**作者**: Zhang L, et al.
**摘要**: 报道了C20orf42在大肠杆菌中的重组表达与纯化策略，并验证其具有ATP酶活性，可能参与核酸代谢或修复通路。
3. **文献名称**: *C20orf42 depletion disrupts neuronal development in vitro*
**作者**: Jones RB, et al.
**摘要**: 利用CRISPR-Cas9技术敲除C20orf42，发现其缺失导致神经细胞分化异常，表明该蛋白在神经发育中具有潜在重要作用。
4. **文献名称**: *Proteomic interaction mapping of C20orf42 reveals association with cilia function*
**作者**: Lee S, et al.
**摘要**: 通过酵母双杂交筛选，发现C20orf42与纤毛组件蛋白相互作用，提示其可能参与纤毛形成或信号传导异常相关疾病。
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**建议**：以上为示例，实际文献需通过PubMed、Web of Science等平台以“C20orf42”“chromosome 20 open reading frame 42”等关键词检索，或结合其已知别名（如若有）扩大搜索范围。
**Background of Recombinant Human C20orf42 Protein**
C20orf42 (Chromosome 20 Open Reading Frame 42), also known as FBXO47, is a predicted protein-coding gene located on human chromosome 20q11.22. The encoded protein belongs to the F-box family, characterized by an F-box domain that typically mediates substrate recognition in ubiquitin ligase complexes (SCF complexes). Despite its identification via genomic sequencing and in silico predictions, the functional characterization of C20orf42 remains limited and primarily inferred from structural homology. Studies suggest potential roles in cellular processes such as protein degradation, cell cycle regulation, or signaling pathways, but experimental validation is sparse.
Notably, F-box proteins like FBXO47 are implicated in substrate-specific ubiquitination, targeting proteins for proteasomal degradation. However, specific interactors or substrates of C20orf42 remain unvalidated. Its expression has been detected in various tissues, with some studies linking altered expression levels to reproductive system development or cancer, though mechanistic insights are lacking. Recombinant forms of C20orf42 are often utilized in biochemical assays (e.g., pull-down experiments, structural studies) to explore its molecular interactions or enzymatic activity. Current research focuses on elucidating its physiological role, including potential involvement in disease pathways, leveraging overexpression, knockdown, or CRISPR-based models. Further functional studies are essential to define its precise biological significance and therapeutic potential.
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