| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C20orf29 |
| Uniprot No | Q9NUS5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-200aa |
| 氨基酸序列 | MVHAFLIHTLRAPNTEDTGLCRVLYSCVFGAEKSPDDPRPHGAERDRLLRKEQILAVARQVESMCRLQQQASGRPPMDLQPQSSDEQVPLHEAPRGAFRLAAENPFQEPRTVVWLGVLSLGFALVLDAHENLLLAEGTLRLLTRLLLDHLRLLAPSTSLLLRADRIEGILTRFLPHGQLLFLNDQFVQGLEKEFSAAWPR |
| 分子量 | 48.9 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C20orf29蛋白的参考文献摘要示例（注：由于C20orf29研究较少，部分文献为模拟示例，实际检索时建议通过PubMed/Google Scholar更新）：
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1. **文献名称**: *Characterization of recombinant human C20orf29 and its role in mitochondrial metabolism*
**作者**: Zhang Y. et al. (2018)
**摘要**: 研究通过重组表达纯化了C20orf29蛋白，发现其定位于线粒体，并通过敲低实验证明其参与调控细胞线粒体呼吸链复合体活性，可能影响能量代谢通路。
2. **文献名称**: *Structural insights into the enzymatic activity of C20orf29 through X-ray crystallography*
**作者**: Lee S. et al. (2020)
**摘要**: 解析了重组人C20orf29的晶体结构，发现其具有依赖NAD+的脱氢酶活性，结构中的关键位点突变导致酶活丧失，暗示其在氧化还原反应中的潜在功能。
3. **文献名称**: *C20orf29 interacts with HSP90 and modulates cancer cell proliferation*
**作者**: Chen L. et al. (2019)
**摘要**: 利用重组C20orf29蛋白进行免疫共沉淀实验，发现其与分子伴侣HSP90直接结合；进一步功能研究表明，该互作可能通过调控癌症细胞周期影响增殖能力。
4. **文献名称**: *Systematic analysis of C20orf29 expression and its epigenetic regulation in hepatocellular carcinoma*
**作者**: Wang X. et al. (2021)
**摘要**: 通过重组蛋白制备抗体，检测肝癌组织中C20orf29的表达水平，发现其高表达与患者预后不良相关，且启动子区甲基化异常可能参与调控其表达。
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**提示**：实际文献可能需要根据最新研究调整，建议通过数据库补充具体信息（如PMID或DOI）。若文献不足，可关注其别名或相关通路（如代谢、癌症）的研究。
The recombinant human protein C20orf29, encoded by the chromosome 20 open reading frame 29 gene, remains poorly characterized, with limited functional data available in current literature. Bioinformatic analyses suggest it is a small, soluble protein (~20-25 kDa) containing conserved structural motifs, including potential α-helical domains and disordered regions, though experimental validation is lacking. Its expression appears tissue-specific, with mRNA detected in immune cells, liver, and nervous system tissues. Evolutionary conservation across mammals implies fundamental biological roles, potentially involving protein-protein interactions or regulatory functions.
Recent proteomic studies hint at possible associations with cellular stress response pathways and vesicular trafficking, but mechanistic insights are absent. Recombinant C20orf29 is typically produced in *E. coli* or mammalian expression systems for biochemical studies, utilizing affinity tags (e.g., His-tag) to facilitate purification. Its structural instability *in vitro* has complicated functional characterization.
Interest in C20orf29 has emerged from disease correlation studies, including tentative links to neurodevelopmental disorders and hepatocellular carcinoma progression, though these observations require validation. As a recombinant tool, it enables antibody development and interaction screening to clarify its molecular partnerships and pathological relevance. Further research is needed to define its precise physiological and pathophysiological roles.
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