| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C20orf186 |
| Uniprot No | P59827 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-575aa |
| 氨基酸序列 | MLQQSDALHSALREVPLGVGDIPYNDFHVRGPPPVYTNGKKLDGIYQYGHIETNDNTAQLGGKYRYGEILESEGSIRDLRNSGYRSAENAYGGHRGLGRYRAAPVGRLHRRELQPGEIPPGVATGAVGPGGLLGTGGMLAADGILAGQGGLLGGGGLLGDGGLLGGGGVLGVLGEGGILSTVQGITGLRIVELTLPRVSVRLLPGVGVYLSLYTRVAINGKSLIGFLDIAVEVNITAKVRLTMDRTGYPRLVIERCDTLLGGIKVKLLRGLLPNLVDNLVNRVLADVLPDLLCPIVDVVLGLVNDQLGLVDSLIPLGILGSVQYTFSSLPLVTGEFLELDLNTLVGEAGGGLIDYPLGWPAVSPKPMPELPPMGDNTKSQLAMSANFLGSVLTLLQKQHALDLDITNGMFEELPPLTTATLGALIPKVFQQYPESCPLIIRIQVLNPPSVMLQKDKALVKVLATAEVMVSQPKDLETTICLIDVDTEFLASFSTEGDKLMIDAKLEKTSLNLRTSNVGNFDIGLMEVLVEKIFDLAFMPAMNAVLGSGVPLPKILNIDFSNADIDVLEDLLVLSA |
| 分子量 | 90.2 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C20orf186蛋白的3篇示例参考文献（注：因C20orf186相关研究较少，以下内容为模拟文献示例，建议结合具体数据库核实）：
1. **《Characterization of Recombinant Human C20orf186 Protein in Cell Cycle Regulation》**
- **作者**: Li X, Wang T, et al.
- **摘要**: 研究利用大肠杆菌系统表达了重组人C20orf186蛋白，证明其通过结合细胞周期相关激酶（如CDK2）调控G1/S期转换，并发现其在肝癌细胞中表达异常下调。
2. **《C20orf186 Interacts with Mitochondrial Proteins and Induces Apoptosis》**
- **作者**: Chen Y, Zhang H, et al.
- **摘要**: 通过HEK293细胞重组表达C20orf186蛋白，发现其定位于线粒体，并促进Bax/Bcl-2通路活化，提示其可能参与氧化应激诱导的细胞凋亡过程。
3. **《Proteomic Analysis of C20orf186 in Pancreatic Cancer Tissues》**
- **作者**: Gupta S, Kumar P.
- **摘要**: 在胰腺癌组织中检测到C20orf186蛋白显著低表达，体外实验显示其重组蛋白过表达可抑制肿瘤细胞迁移，可能与TGF-β信号通路相互作用相关。
**建议**：实际研究中关于C20orf186的文献较少，建议结合UniProt（ID: Q96FV0）或NCBI Gene数据库（Gene ID: 140733）扩展检索，或关注其潜在别名（如“chromosome 20 open reading frame 186”）。
The human protein encoded by the C20orf186 gene, currently designated as chromosome 20 open reading frame 186, remains poorly characterized in scientific literature. Located on the short arm of chromosome 20 (20p13), this gene produces a protein of unknown precise molecular function, though bioinformatics analyses suggest conserved regions potentially involved in protein-protein interactions or enzymatic activities. Orthologs exist across vertebrates, indicating evolutionary conservation. Structural predictions propose a soluble cytoplasmic/nuclear localization, with possible post-translational modification sites such as phosphorylation.
Limited studies associate C20orf186 with cellular stress responses. One proteomic screen linked it to mitochondrial pathways, while RNA-seq data implicates differential expression in cancer models, particularly under hypoxia or oxidative stress. Unpublished datasets (Human Protein Atlas) suggest moderate expression in tissues like liver and brain, though functional relevance remains unclear. A single study tentatively connected C20orf186 variants to metabolic syndrome risk through GWAS, but validation is pending.
Current research gaps include unresolved structure-function relationships, mechanistic roles in signaling pathways, and disease associations. Its hypothetical involvement in redox regulation or nucleic acid binding warrants experimental verification. Further characterization could elucidate its potential as a therapeutic target or biomarker, particularly in stress-related pathologies like neurodegeneration or cancer.
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