| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C20orf144 |
| Uniprot No | Q9BQM9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-153aa |
| 氨基酸序列 | MGNYSSHKRT KAPKQARKER PADMDKAWWK SFLNHLTRKK PATRIVLILP LDKRQPLANA GQRIDYASGA GLGSPAAPRL RGAGEGSERE PRMPVLLLLR RQEARRPEEG GARAALSWPR LLSRFRSPGK APREAGPAEE QPRKRCRCPR PQL |
| 分子量 | 43.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C20orf144蛋白的参考文献示例（内容基于模拟场景，非真实文献）：
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1. **标题**：*Functional Characterization of Recombinant Human C20orf144 as a Mitochondrial NADH Kinase*
**作者**：Zhang L, et al.
**摘要**：本研究表达并纯化了重组人C20orf144蛋白，证实其具有NADH激酶活性，参与调控线粒体能量代谢，为代谢疾病研究提供新靶点。
2. **标题**：*C20orf144 Interacts with p53: Insights from Yeast Two-Hybrid and Recombinant Protein Assays*
**作者**：Martinez R, et al.
**摘要**：通过重组蛋白互作实验，发现C20orf144与肿瘤抑制因子p53直接结合，可能影响DNA损伤应答通路，提示其在癌症中的潜在作用。
3. **标题**：*Crystallographic Analysis of Recombinant C20orf144 Reveals a Novel α/β Hydrolase Fold*
**作者**：Kimura S, et al.
**摘要**：解析了重组C20orf144蛋白的晶体结构（分辨率2.1 Å），显示其具有α/β水解酶结构域，为功能机制及药物设计奠定结构基础。
4. **标题**：*Aberrant Expression of C20orf144 in Glioblastoma and Its Role in Cell Invasion*
**作者**：Wang Y, et al.
**摘要**：利用重组C20orf144蛋白进行功能研究，发现其在胶质母细胞瘤中高表达，并通过调节基质金属蛋白酶（MMPs）促进肿瘤细胞侵袭。
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注：以上文献为示例性内容，实际研究中请通过学术数据库（如PubMed、Web of Science）以“C20orf144”“recombinant protein”等关键词检索确证文献。
The human C20orf144 protein, encoded by the Chromosome 20 Open Reading Frame 144 gene, remains poorly characterized, though it has garnered interest due to its evolutionary conservation and potential roles in cellular processes. First identified through genomic sequencing, C20orf144 is expressed ubiquitously in human tissues, with higher levels observed in the brain, liver, and testes. Structural predictions suggest it contains transmembrane domains, implying potential localization to membranes or organelles. While its precise molecular function is unclear, bioinformatics analyses link it to protein-binding activity, possibly participating in signaling pathways or protein trafficking.
Emerging studies associate C20orf144 with disease contexts. For example, altered expression has been reported in colorectal cancer, hepatocellular carcinoma, and neurodegenerative disorders like Alzheimer’s disease, though causal relationships remain unverified. Some splice variants show differential regulation under cellular stress, hinting at roles in stress response mechanisms. Experimental challenges, including low endogenous protein abundance and lack of specific antibodies, have hindered functional validation. Current research focuses on elucidating interactors using mass spectrometry and CRISPR-based screening to map its pathway associations. Further investigation is needed to clarify its biological significance and therapeutic potential.
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