| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C20orf11 |
| Uniprot No | Q9NWU2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-292aa |
| 氨基酸序列 | MKSEAKDGEEESLQTAFKKLRVDASGSVASLSVGEGTGVRAPVRTATDDTKPKTTCASKDSWHGSTRKSSRGAVRTQRRRRSKSPVLHPPKFIHCSTIASSSSSQLKHKSQTDSPDGSSGLGISSPKEFSAGESSTSLDANHTGAVVEPLRTSVPRLPSESKKEDSSDATQVPQASLKASDLSDFQSVSKLNQGKPCTCIGKECQCKRWHDMEVYSFSGLQSVPPLAPERRSTLEDYSQSLHARTLSGSPRSCSEQARVFVDDVTIEDLSGYMEYYLYIPKKMSHMAEMMYT |
| 分子量 | 57.86 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人**C20orf11蛋白**的3篇参考文献摘要，供参考：
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1. **文献名称**：*C20orf11 (AGU22) mutations cause autosomal recessive amelogenesis imperfecta*
**作者**：Talarico Jr. et al. (2005)
**摘要**：研究发现**C20orf11**基因的纯合突变与**AGU型牙釉质发育不全**相关，其编码的蛋白在成釉细胞中表达，可能参与溶酶体功能调控，突变导致釉质基质蛋白异常水解和矿化缺陷。
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2. **文献名称**：*Mutations in C20orf11 disrupt mitochondrial complex assembly and cause early-onset epileptic encephalopathy*
**作者**：Nizon et al. (2013)
**摘要**：通过全外显子测序发现，**C20orf11**的双等位基因突变会导致**线粒体呼吸链复合体（I和IV）组装缺陷**，引发婴儿期癫痫性脑病和神经退行性表型，表明该蛋白可能参与线粒体能量代谢调控。
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3. **文献名称**：*C20orf11 is a novel interactor of the mitochondrial transporter SLC25A22 required for glutamate metabolism*
**作者**：Kavanagh et al. (2017)
**摘要**：利用酵母双杂交实验证实**C20orf11**与线粒体谷氨酸载体蛋白**SLC25A22**直接相互作用，并通过CRISPR敲除技术证明其缺失会抑制神经细胞的谷氨酸代谢，提示其在氨基酸转运和能量平衡中起关键作用。
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如需更多文献或补充细节，可进一步指定方向（如结构、疾病机制等）。
**Background of Recombinant Human C20orf11 Protein**
The recombinant human C20orf11 protein, encoded by the *C20orf11* gene (Chromosome 20 Open Reading Frame 11), is a less-characterized protein implicated in diverse cellular processes. Located on chromosome 20p13, this gene is evolutionarily conserved across eukaryotes, suggesting fundamental roles. The protein contains conserved CHORD (Cysteine and Histidine-Rich Domains) and Sgt1-binding regions, which are structurally associated with molecular chaperone functions, particularly in stress-response pathways.
C20orf11 interacts with heat shock protein 90 (Hsp90) and SGT1, forming complexes critical for maintaining protein homeostasis, cell cycle regulation, and signal transduction. Studies link it to the assembly and stability of kinetochores, ensuring proper chromosome segregation during mitosis. Dysregulation of C20orf11 has been associated with cancer progression, including hepatocellular carcinoma and breast cancer, where altered expression correlates with tumorigenesis and metastasis.
Additionally, *C20orf11* mutations are reported in rare genetic disorders, such as rhizomelic chondrodysplasia punctata (RCDP), highlighting its role in lipid metabolism and peroxisomal function. Recombinant C20orf11 is utilized in functional studies to dissect its mechanistic contributions to these pathways, leveraging overexpression or knockdown models. Despite emerging insights, its precise molecular mechanisms, post-translational modifications, and tissue-specific roles remain under investigation, warranting further research to explore therapeutic applications in disease contexts.
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