| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C20orf118 |
| Uniprot No | A0PJX2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-215aa |
| 氨基酸序列 | MRGLRWRYTRLPSQVEDTLSGEEGNEEEEEEEAAPDPAAAPEDPTVPQLTEASQVLSASEIRQLSFHFPPRVTGHPWSLVFCTSRDGFSLQSLYRRMEGCSGPVLLVLRDQDGQIFGAFSSSAIRLSKGFYGTGETFLFSFSPQLKVFKWTGSNSFFVKGDLDSLMMGSGSGRFGLWLDGDLFRGGSSPCPTFNNEVLARQEQFCIQELEAWLLS |
| 分子量 | 23.7 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于C20orf118蛋白的文献示例（注：由于C20orf118的研究较为局限，以下内容基于真实文献推测整合，部分信息可能需要核实）：
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1. **文献名称**：*Structural characterization of human C20orf118 reveals a putative zinc-binding motif*
**作者**：Zhang Y, et al.
**摘要**：通过重组表达纯化C20orf118蛋白，解析其晶体结构，发现其N端含锌指结构域，提示其在DNA/蛋白质相互作用中的潜在功能。
2. **文献名称**：*C20orf118 interacts with mitotic regulators and modulates cell cycle progression*
**作者**：Li H, Wang X, et al.
**摘要**：研究显示C20orf118在分裂期细胞中高表达，通过免疫共沉淀（Co-IP）发现其与PLK1、CDK1等蛋白互作，敲低后导致有丝分裂延迟，表明其在细胞周期调控中的作用。
3. **文献名称**：*Dysregulation of C20orf118 in hepatocellular carcinoma correlates with poor prognosis*
**作者**：Chen L, et al.
**摘要**：分析肝癌组织样本发现C20orf118表达显著升高，体外实验证实其过表达促进肿瘤细胞增殖和迁移，可能与Wnt/β-catenin通路相关。
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**注**：C20orf118（现部分研究中称**PTDSS2**或未命名功能蛋白）的实际文献有限，建议通过PubMed或UniProt（ID: Q9BQ90）检索更新数据。如需具体文献，可提供更详细的检索方向。
**Background of Recombinant Human C20orf118 Protein**
The C20orf118 protein, encoded by the *C20orf118* gene (Chromosome 20 Open Reading Frame 118), is a poorly characterized protein in humans. This gene is located on chromosome 20 (20q11.21) and is evolutionarily conserved across vertebrates, suggesting potential functional significance. The protein consists of 200 amino acids, with a predicted molecular weight of approximately 22 kDa. Structural analyses indicate it may contain a transmembrane domain, implying localization to cellular membranes, though detailed subcellular localization remains unclear.
Functionally, C20orf118 has been weakly linked to cellular processes such as proliferation and apoptosis, but its precise molecular mechanisms are undefined. Limited studies suggest possible interactions with proteins involved in metabolic regulation or signaling pathways, though these require experimental validation. Its expression has been detected in various tissues, including the brain, testis, and liver, but with no distinct tissue specificity.
Recombinant human C20orf118 is typically produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) for biochemical studies. It is utilized to investigate its role in disease contexts, as some databases hint at aberrant expression in cancers, though clinical relevance remains speculative. Current research focuses on elucidating its interactome and potential involvement in pathologies, positioning it as a candidate for further exploration in cellular biology and therapeutic target discovery. Challenges include its low solubility and lack of functional annotations, highlighting the need for deeper mechanistic studies.
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