关键信息
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基因名
gABRa5
- 应用
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别名
gABRa5；Gamma-aminobutyric acid receptor subunit alpha-5
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P31644
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表达区间
1-462aa
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氨基酸序列
MDNGMFSGFIMIKNLLLFCISMNLSSHFGFSQMPTSSVKDETNDNITIFTRILDGLLDGYDNRLRPGLGERITQVRTDIYVTSFGPVSDTEMEYTIDVFFRQSWKDERLRFKGPMQRLPLNNLLASKIWTPDTFFHNGKKSIAHNMTTPNKLLRLEDDGTLLYTMRLTISAECPMQLEDFPMDAHACPLKFGSYAYPNSEVVYVWTNGSTKSVVVAEDGSRLNQYHLMGQTVGTENISTSTGEYTIMTAHFHLKRKIGYFVIQTYLPCIMTVILSQVSFWLNRESVPARTVFGVTTVLTMTTLSISARNSLPKVAYATAMDWFIAVCYAFVFSALIEFATVNYFTKRGWAWDGKKALEAAKIKKKREVILNKSTNAFTTGKMSHPPNIPKEQTPAGTSNTTSVSVKPSEEKTSESKKTYNSISKIDKMSRIVFPVLFGTFNLVYWATYLNREPVIKGAASPK
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分子量
52.1 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
The study of the GABRA5 recombinant protein has gained considerable attention in the field of neuroscience and pharmacology due to its critical role in the function of GABA(A) receptors, which are essential for inhibitory neurotransmission in the central nervous system. GABRA5 is a subunit of these receptors and its specific interactions significantly influence the receptor's pharmacological properties, including sensitivity to various ligands such as anesthetics, anxiolytics, and sedatives. Understanding the structure and function of GABRA5 can provide valuable insights into the mechanisms underlying several neurological and psychiatric disorders, such as anxiety, depression, epilepsy, and schizophrenia. Moreover, alterations in the expression or function of GABRA5 have been implicated in various pathophysiological conditions, highlighting the importance of this subunit in therapeutic research. By utilizing recombinant DNA technology, researchers can produce GABRA5 in a controlled manner, enabling detailed studies of its biochemical properties, interaction with other subunits, and the effects of specific mutations. This knowledge may ultimately contribute to the development of novel pharmacological agents targeting GABA(A) receptors to modulate their activity for therapeutic benefits in managing neuropsychiatric disorders. This research is critical not only for basic neuroscience but also for potential clinical applications aimed at enhancing mental health and treating related conditions.
