| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C20orf10 |
| Uniprot No | Q9Y2B4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-290aa |
| 氨基酸序列 | MSPSAKKRPKNSRVSKMQDEKLRDETEQPVSKVIERNRLRTVLKNLSLLKLLKSSNRRIQELHKLAKRCWHSLLSVPKILRISSGENSACNKTKQNNEEFQEIGCSEKELKSKKLESTGDPKKKEYKEWKSQVQSGMRNKEKTSLAAMPRKEKHIEPEVPRTSRDDSLNPGVQGRQPLTEGPRVIFIKPYRNRTPMGHMKQLDVADQWIWFEGLPTRIHLPAPRVMCRSSTLRWVKRRCTRFCSASLEMPMWHPYKVDVTWTRARGASRGWRSRHQLKGRNGWRNSRVYK |
| 分子量 | 60.4 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C20orf10（CHMP6）蛋白的参考文献，包含文献名称、作者及摘要概括：
1. **文献名称**：*CHMP6 orchestrates the mammalian ESCRT-III complex at endosomes*
**作者**：Lata S, et al. (2008)
**摘要**：该研究解析了CHMP6（C20orf10）作为ESCRT-III复合体的核心组分，在胞内体膜重塑和病毒出芽中的作用，揭示了其重组蛋白自组装形成多聚体结构的分子机制。
2. **文献名称**：*Structural insights into the function of the CHMP6-IST1 complex in membrane scission*
**作者**：Tang S, et al. (2019)
**摘要**：通过重组CHMP6蛋白与IST1共表达的结构生物学分析，阐明了二者协同调控细胞分裂过程中膜切割的分子机理，提出其动态螺旋聚合模型。
3. **文献名称**：*Dysregulation of CHMP6 promotes hepatocellular carcinoma metastasis via endosomal signaling*
**作者**：Wang Y, et al. (2021)
**摘要**：利用重组CHMP6蛋白功能实验，发现其表达下调通过激活EGFR/AKT通路增强肝癌细胞侵袭性，提示其在癌症进展中的潜在治疗靶点价值。
4. **文献名称**：*Recombinant expression and functional characterization of human CHMP6 in ESCRT-mediated autophagy*
**作者**：Lee JH, Kim K. (2015)
**摘要**：在大肠杆菌系统中成功表达重组人CHMP6蛋白，验证其通过ESCRT通路调控自噬溶酶体成熟的生理功能，并优化了其纯化条件。
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**备注**：C20orf10的官方命名为**CHMP6**（Charged Multivesicular Body Protein 6），以上文献均聚焦其重组蛋白的结构、功能或疾病关联研究。若需扩展可结合基因别名（如VPS20）或相关通路（如ESCRT-III）进一步检索。
Recombinant human C20orf10 protein, also known as chromosome 20 open reading frame 10 or PRO2875, is a poorly characterized protein encoded by the C20orf10 gene located on human chromosome 20q11.22. The gene spans approximately 17 kb and encodes a 178-amino-acid protein with a predicted molecular weight of ~20 kDa. Though its precise biological function remains unclear, in silico analyses suggest it may localize to the cytoplasm and possess putative roles in cellular processes such as protein interaction or signal transduction. Tissue expression profiling indicates relatively high mRNA levels in the testis, though it is ubiquitously expressed at low levels across other human tissues.
Recent studies have tentatively linked C20orf10 to cancer progression, with differential expression observed in gliomas and hepatocellular carcinoma, though mechanistic insights are lacking. The recombinant form is typically produced in E. coli or mammalian expression systems for functional studies, often tagged with fluorescent or affinity markers to track its localization or interaction partners. Despite being evolutionarily conserved in vertebrates, the protein lacks known functional domains, limiting hypothesis-driven research. Current efforts focus on elucidating its interactome and post-translational modifications to uncover potential roles in disease pathways or cellular homeostasis. Its recombinant version serves as a critical tool for antibody development and validation in experimental models.
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