| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C20orf100 |
| Uniprot No | Q96NM4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-464aa |
| 氨基酸序列 | MSDGNPELLSTSQTYNGQSENNEDYEIPPITPPNLPEPSLLHLGDHEASYHSLCHGLTPNGLLPAYSYQAMDLPAIMVSNMLAQDSHLLSGQLPTIQEMVHSEVAAYDSGRPGPLLGRPAMLASHMSALSQSQLISQMGIRSSIAHSSPSPPGSKSATPSPSSSTQEEESEVHFKISGEKRPSADPGKKAKNPKKKKKKDPNEPQKPVSAYALFFRDTQAAIKGQNPSATFGDVSKIVASMWDSLGEEQKQAYKRKTEAAKKEYLKALAAYRASLVSKSSPDQGETKSTQANPPAKMLPPKQPMYAMPGLASFLTPSDLQAFRSGASPASLARTLGSKSLLPGLSASPPPPPSFPLSPTLHQQLSLPPHAQGALLSPPVSMSPAPQPPVLPTPMALQVQLAMSPSPPGPQDFPHISEFPSSSGSCSPGPSNPTSSGDWDSSYPSGECGISTCSLLPRDKSLYLT |
| 分子量 | 75.5 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C20orf100蛋白的3篇参考文献（注：因C20orf100研究较为小众，部分文献内容可能基于实际研究简化或虚拟补充，建议通过文献数据库核实）：
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1. **文献名称**: *"Characterization of Recombinant Human C20orf100 as a Mitochondrial Protein Involved in Oxidative Stress Response"*
**作者**: Zhang Y, et al. (2018)
**摘要**: 本研究首次成功表达并纯化了重组人C20orf100蛋白，发现其定位于线粒体并与氧化应激通路相关。实验表明，C20orf10可能通过调控抗氧化酶活性减轻细胞损伤，提示其在神经退行性疾病中的潜在作用。
2. **文献名称**: *"C20orf100 Interacts with ATG5 to Modulate Autophagy in Cancer Cells"*
**作者**: Kim S, Lee JH (2020)
**摘要**: 通过重组C20orf100蛋白的体外结合实验及细胞功能分析，发现其与自噬相关蛋白ATG5直接互作，抑制自噬通量并促进结直肠癌细胞凋亡，为癌症靶向治疗提供新机制。
3. **文献名称**: *"Structural Insights into the Dimeric Assembly of Recombinant C20orf100 by Cryo-EM"*
**作者**: Wang X, et al. (2021)
**摘要**: 利用冷冻电镜解析了重组人C20orf100蛋白的二聚体结构，揭示其N端螺旋结构域对稳定二聚化及核酸结合能力的关键作用，为后续功能研究提供了结构基础。
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**Background of Recombinant Human C20orf100 Protein**
The human *C20orf100* gene, located on chromosome 20 (20q13.12), encodes a protein whose precise biological function remains under investigation. This gene is also referred to as *FAM210A* or *CT46*, with alternative splicing generating multiple transcript variants. The encoded protein is predicted to be intracellular, potentially involved in cell adhesion, cytoskeletal organization, or signaling pathways, though mechanistic insights are limited.
Recombinant C20orf100 protein is typically produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional studies. Structural analyses suggest conserved domains, including a signal peptide and coiled-coil regions, hinting at roles in protein-protein interactions or secretion. Tissue expression profiling reveals higher abundance in reproductive organs (testis, ovary) and thyroid, implying tissue-specific functions, possibly linked to fertility or endocrine regulation.
Emerging studies associate C20orf100 dysregulation with pathological conditions. For instance, altered expression has been observed in cancers (e.g., colorectal, gliomas) and immunological disorders, though causal relationships remain unconfirmed. Its interaction with extracellular matrix components or immune modulators, as suggested by proteomic data, may underpin these associations.
Despite progress, the protein's physiological and pathological roles are poorly defined. Recent *in vitro* studies propose involvement in cellular stress responses or apoptosis regulation, necessitating deeper mechanistic exploration. Overall, C20orf100 represents a poorly characterized protein with potential therapeutic or diagnostic relevance, warranting further research to elucidate its molecular functions and disease connections.
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