| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C1orf96 |
| Uniprot No | Q6IQ19 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-140aa |
| 氨基酸序列 | MSPGSGVKSEYMKRYQEPRWEEYGPCYRELLHYRLGRRLLEQAHAPWLWDDWGPAGSSEDSASSESSGAGGPAPRCAPPSPPPPVEPATQEEAERRARGAPEEQDAEAGDAEAEDAEDAALPGTRPGGEREGPARRDGLL |
| 分子量 | 41.6 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为基于常见研究方向的虚构参考文献示例，供参考（需注意实际文献需通过学术数据库验证）：
1. **Smith, J. et al. (2020).**
*"Functional Analysis of Recombinant C1orf96 in Cell Cycle Regulation."*
摘要：通过重组表达C1orf96蛋白，研究发现其与细胞周期蛋白Cyclin D1存在相互作用，可能通过调控G1/S期转换促进肿瘤细胞增殖。
2. **Li, X. et al. (2019).**
*"Expression and Purification of Recombinant Human C1orf96 for Antibody Development."*
摘要：利用大肠杆菌系统成功表达重组C1orf96，并制备多克隆抗体，免疫组化显示其在多种癌组织中高表达，提示潜在诊断价值。
3. **Garcia-Ruiz, S. et al. (2021).**
*"C1orf96 Knockdown Impairs Mitochondrial Function via Recombinant Protein Interaction Studies."*
摘要：通过重组C1orf96与线粒体复合物的体外结合实验，揭示其可能参与能量代谢调控，基因沉默导致细胞ATP水平显著下降。
4. **Chen, L. et al. (2018).**
*"Structural Insights into the Uncharacterized Protein C1orf96 by Recombinant Expression and Crystallography."*
摘要：首次解析重组C1orf96的晶体结构，揭示其含有保守的α/β折叠域，推测可能作为信号转导中的适配分子。
**注意：上述文献为示例性质，实际研究中请通过学术平台（如PubMed、Google Scholar）以"C1orf96"为关键词检索最新成果。未表征蛋白的研究常涉及基础表达分析、相互作用筛选或疾病关联探索。**
C1orf96, also termed Chromosome 1 Open Reading Frame 96, is a poorly characterized human protein encoded by the C1orf96 gene located on chromosome 1p36.13. Despite its discovery through genomic sequencing, its molecular function, physiological role, and interactome remain largely unknown. The protein is predicted to be small (~20 kDa), with limited conserved domains beyond a putative coiled-coil motif, suggesting possible roles in protein-protein interactions. Bioinformatics analyses indicate low evolutionary conservation, hinting at species-specific functions in humans. Expression profiling reveals variable mRNA levels across tissues, with higher abundance in the testis, suggesting potential relevance to reproductive biology. Some studies associate C1orf96 with cancers, particularly breast and lung carcinomas, where its overexpression correlates with poor prognosis. Preliminary evidence links it to cellular proliferation and apoptosis regulation, though mechanistic insights are lacking. Recent interest stems from its identification in proteomic screens of stress granules and chromatin-associated complexes, implying roles in nucleic acid metabolism or stress response. Recombinant C1orf96 protein production (e.g., in E. coli) facilitates antibody development and structural studies, though purification challenges persist due to intrinsic disorder predictions. Ongoing research focuses on validating interaction partners via yeast two-hybrid systems and mass spectrometry. Its classification as a "dark protein" underscores the need for functional characterization to clarify its contribution to human physiology and disease pathways.
×
