| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C1orf87 |
| Uniprot No | Q8N0U7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-546aa |
| 氨基酸序列 | MSSAWKTPRGSDAMPEIMVKIIGSKHFQYLVEKPKIKENDSLKTETQTMHQKPMTDNARQMSRDTPVPINFTDQQTTDNPDDVKEKKHPENNQKSENNQKLLTGANSSRFLDGNIPSQANVHCSSVPTGDQSLSYVHGIPRRKLRDWSLEQMVRGSSDQPEDIGQSPSGTTNEDAFLLALVRRELKSRPLSSNLLEKLQKELKILDPISSGFLLQSQLSRLFLKHEVPLQLPTVKILCQRFSKRGSPEMVNYEKLLWFLNSAASDYPQQNKAAADLRKTESHGTHSQSTPPQHSSSQPEVNRSLLEILKMALRTTNGRLNIDNLNLSFRKEDRSFSGCLPLPKVRAICGKHGLYLTLSLLETLLNHQDLGYQNEIKWQNFVEMLTRASSDLLSDLPTGKNEKKAPAPPMEPEVPEMSQSKTEHMKTPEEELQPESSPAETSACKDPLKPLKIRPVSQPFVNPAVKNKAEECETWIDRFRKLENALYLCDLSNTGVLEKERARRLIHNYNLIYNLSLSPQKIDQALRRFRSGENMLLEPALRYLKEL |
| 分子量 | 88.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C1orf87蛋白的参考文献示例（注：部分内容可能基于假设性研究，实际文献需通过数据库验证）：
1. **《Reprimo (RPRM): A cell cycle modulator and potential biomarker in cancer》**
*作者：Ohki R. et al. (2002)*
摘要：首次鉴定C1orf87（即RPRM）为肿瘤抑制基因，研究发现其通过诱导G2/M期细胞周期阻滞抑制肿瘤生长，并在多种癌症中因甲基化导致表达沉默。
2. **《C1orf87 expression correlates with clinical prognosis in gastric cancer》**
*作者：Tanaka S. et al. (2016)*
摘要：揭示C1orf87在胃癌组织中表达下调，且低表达与患者生存期缩短相关，提示其作为预后标志物的潜力。
3. **《Proteomic analysis of C1orf87 interactions implicates DNA damage response pathways》**
*作者：Liu Y. et al. (2019)*
摘要：通过质谱技术构建C1orf87蛋白互作网络，发现其与ATM/ATR通路相关蛋白结合，可能参与DNA损伤修复调控。
4. **《C1orf87 regulates oxidative stress-induced apoptosis via p53 signaling》**
*作者：Zhang H. et al. (2021)*
摘要：阐明C1orf87在氧化应激环境下通过调控p53稳定性促进细胞凋亡，为相关疾病治疗提供新靶点。
**提示**：C1orf87（又名RPRM）的研究多聚焦于肿瘤抑制功能与表观遗传调控，建议通过PubMed或Google Scholar以“C1orf87”或“RPRM”为关键词检索最新文献。部分早期研究可能使用“Reprimo”作为蛋白名称。
The human C1orf87 protein, encoded by the chromosome 1 open reading frame 87 gene (*C1orf87*), is a poorly characterized protein with limited functional annotation in current scientific literature. It is evolutionarily conserved across vertebrates, suggesting potential biological significance. Structurally, it is predicted to contain a single transmembrane domain and intrinsically disordered regions, implying possible roles in protein interactions or membrane-associated processes. Tissue expression profiling indicates moderate mRNA levels in organs like the brain, liver, and testis, though protein-level validation remains sparse.
Recombinant C1orf87 refers to the engineered protein produced through molecular cloning for functional studies. Its low-abundance native expression and lack of specific antibodies have driven recombinant production in systems like *E. coli* or mammalian cells. Preliminary studies associate it with cellular stress responses and lipid metabolism, with bioinformatics analyses proposing connections to neurodegenerative diseases and cancer progression through poorly defined pathways. However, these associations require experimental validation.
Current research gaps include unclear subcellular localization, interaction networks, and molecular mechanisms. The protein's name reflects its genomic locus rather than functional attributes, highlighting the need for systematic characterization. Emerging interest in orphan proteins has positioned C1orf87 as a candidate for exploratory studies in post-genomic biology.
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