| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C1orf84 |
| Uniprot No | Q5T011 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-178aa |
| 氨基酸序列 | MASERPEPEVEEAGQVFLLMKKDYRISRNVRLAWFLSHLHQTVQATPQEMLSEQELEVLSVLPPGWQPDEPVVPRPFLLVPSTRVTFLAWQYRFVIELDLSPSTGIVDDSTGEILFDEVFHALSRCLGGLLRPFRVPGSCIDFQPEIYVTIQAYSSIIGLQSHQRQGFTMLARLPSNF |
| 分子量 | 46.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C1orf84蛋白的参考文献示例（虚构内容，仅供参考）：
1. **文献名称**：Structural and functional analysis of human C1orf84 protein
**作者**：Zhang Y, et al.
**摘要**：本研究成功表达了重组人C1orf84蛋白，通过X射线晶体学解析其三维结构，并揭示其可能的ATP结合活性，提示其在细胞能量代谢中的作用。
2. **文献名称**：C1orf84 interacts with mitochondrial proteins: implications for apoptosis regulation
**作者**：Lee S, et al.
**摘要**：通过重组C1orf84蛋白的免疫共沉淀实验，发现其与Bcl-2家族蛋白互作，可能在癌细胞凋亡通路中发挥调控功能。
3. **文献名称**：Recombinant C1orf84 expression in E. coli and its role in DNA repair
**作者**：Johnson R, et al.
**摘要**：利用大肠杆菌系统高效表达可溶性C1orf84重组蛋白，实验证实其结合DNA损伤位点，可能参与碱基切除修复机制。
4. **文献名称**：C1orf84 as a biomarker in hepatocellular carcinoma: a proteomic study
**作者**：Wang H, et al.
**摘要**：基于重组C1orf84的抗体制备，发现其在肝癌组织中显著高表达，提示其作为潜在诊断标志物的价值。
（注：上述内容为模拟生成，实际文献需通过学术数据库检索验证。）
C1orf84, also known as chromosome 1 open reading frame 84, is a human protein encoded by the C1orf84 gene located on the long arm of chromosome 1 (1q24). Its biological role remains poorly characterized, but emerging studies suggest it may function as a secreted glycoprotein involved in extracellular signaling or cell-cell interactions. Structural predictions indicate the presence of an N-terminal signal peptide, multiple N-glycosylation sites, and conserved cysteine-rich motifs resembling epidermal growth factor (EGF)-like domains, which are often implicated in protein-protein binding.
Limited experimental evidence links C1orf84 to cellular adhesion, proliferation, and differentiation processes, with potential associations to Wnt or TGF-β signaling pathways. Expression profiling shows its mRNA is detectable in various tissues, including the prostate, brain, and reproductive organs. Notably, altered C1orf84 expression has been observed in certain cancers, such as prostate adenocarcinoma and gliomas, suggesting a possible role in tumor progression or as a diagnostic marker. However, these findings require further validation.
Recombinant C1orf84 protein is typically produced using mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper glycosylation. Current research focuses on elucidating its structure-function relationships, interactome, and therapeutic potential. Despite its enigmatic nature, C1orf84 represents a novel target for studying cellular communication mechanisms and disease pathways.
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