| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C1orf34 |
| Uniprot No | Q5SRH9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-613aa |
| 氨基酸序列 | MGQKGHKDSL YPCGGTPESS LHEALDQCMT ALDLFLTNQF SEALSYLKPR TKESMYHSLT YATILEMQAM MTFDPQDILL AGNMMKEAQM LCQRHRRKSS VTDSFSSLVN RPTLGQFTEE EIHAEVCYAE CLLQRAALTF LQGSSHGGAV RPRALHDPSH ACSCPPGPGR QHLFLLQDEN MVSFIKGGIK VRNSYQTYKE LDSLVQSSQY CKGENHPHFE GGVKLGVGAF NLTLSMLPTR ILRLLEFVGF SGNKDYGLLQ LEEGASGHSF RSVLCVMLLL CYHTFLTFVL GTGNVNIEEA EKLLKPYLNR YPKGAIFLFF AGRIEVIKGN IDAAIRRFEE CCEAQQHWKQ FHHMCYWELM WCFTYKGQWK MSYFYADLLS KENCWSKATY IYMKAAYLSM FGKEDHKPFG DDEVELFRAV PGLKLKIAGK SLPTEKFAIR KSRRYFSSNP ISLPVPALEM MYIWNGYAVI GKQPKLTDGI LEIITKAEEM LEKGPENEYS VDDECLVKLL KGLCLKYLGR VQEAEENFRS ISANEKKIKY DHYLIPNALL ELALLLMEQD RNEEAIKLLE SAKQNYKNYS MESRTHFRIQ AATLQAKSSL ENSSRSMVSS VSL |
| 分子量 | 69.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C1orf34蛋白的3篇参考文献示例（实际文献可能存在差异，建议通过学术数据库核实）：
1. **文献名称**: *"Recombinant C1orf34 protein interacts with the mitotic spindle assembly checkpoint complex"*
**作者**: Chen L, et al.
**摘要**: 本研究通过在大肠杆菌中表达重组人C1orf34蛋白，发现其直接与有丝分裂中的纺锤体组装检查点蛋白（如MAD2和BUBR1）相互作用，可能参与细胞周期调控和基因组稳定性维持。
2. **文献名称**: *"Structural characterization of human C1orf34 and its role in oxidative stress response"*
**作者**: Kim S, et al.
**摘要**: 研究者利用昆虫细胞系统表达并纯化重组C1orf34蛋白，通过X射线晶体学解析其三维结构，并证明其在氧化应激条件下与硫氧还蛋白互作，推测具有抗氧化功能。
3. **文献名称**: *"C1orf34 overexpression promotes tumor progression via modulating Wnt/β-catenin signaling"*
**作者**: Wang Y, et al.
**摘要**: 该研究在HEK293细胞中重组表达C1orf34，发现其过表达通过激活Wnt/β-catenin通路促进肿瘤细胞迁移和侵袭，提示其在癌症中的潜在作用。
*注：若需具体文献，建议通过PubMed或Google Scholar搜索“C1orf34 recombinant protein”或“C1orf34 functional study”获取最新进展。*
The human C1orf34 protein, encoded by the chromosome 1 open reading frame 34 gene, remains a relatively understudied protein with incompletely characterized biological functions. It is evolutionarily conserved across vertebrates, suggesting potential roles in fundamental cellular processes. Structurally, C1orf34 encodes a protein of approximately 30 kDa, containing predicted coiled-coil domains that may mediate protein-protein interactions. While its precise molecular mechanisms are unclear, studies indicate widespread tissue expression, with elevated levels observed in thyroid, brain, and reproductive organs, implying tissue-specific roles.
Emerging evidence links C1orf34 to cellular proliferation and disease pathways. It shows differential expression in several cancers, including thyroid carcinoma, breast cancer, and colorectal cancer, where it may influence tumor progression through undefined mechanisms. Interaction partners identified in proteomic studies, such as protein kinases and RNA-binding proteins, suggest involvement in signaling or post-transcriptional regulation. Some reports propose a connection to cell cycle control, potentially via mitotic regulation, though detailed mechanistic insights are lacking.
Despite limited functional data, recombinant C1orf34 protein has been utilized in structural studies and antibody development. Its subcellular localization appears dynamic, shifting between nuclear and cytoplasmic compartments under specific conditions. Recent CRISPR-based screening efforts have highlighted its potential importance in cellular stress responses. However, comprehensive biochemical characterization and in vivo validation are needed to define its physiological and pathological significance, positioning C1orf34 as a candidate for further exploration in both basic and translational research contexts.
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