| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C1orf192 |
| Uniprot No | Q5VTH2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-177aa |
| 氨基酸序列 | MATNYSANQYEKAFSSKYLQNWSPTKPTKESISSHEGYTQIIANDRGHLLPSVPRSKANPWGSFMGTWQMPLKIPPARVTLTSRTTAGAASLTKWIQKNPDLLKASNGLCPEILGKPHDPDSQKKLRKKSITKTVQQARSPTIIPSSPAANLNSPDELQSSHPSAGHTPGPQRPAKS |
| 分子量 | 45.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
由于C1orf192蛋白的研究较为有限，以下为假设性参考文献示例，供参考格式（实际文献可能需要进一步检索确认）：
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1. **"Recombinant expression and functional analysis of C1orf192 in DNA repair pathways"**
*Author: Chen et al.*
**摘要**: 本研究成功在大肠杆菌系统中表达并纯化了重组人C1orf192蛋白，通过体外实验证明其与DNA损伤修复复合物的相互作用，提示其潜在功能参与基因组稳定性调控。
2. **"C1orf192: A novel mitochondrial protein linked to oxidative stress response"**
*Author: Müller et al.*
**摘要**: 利用重组C1orf192蛋白进行细胞定位实验，发现其定位于线粒体，并可能在氧化应激条件下调节ROS清除相关酶的活性。
3. **"Proteomic screening of C1orf192-binding partners using recombinant affinity tags"**
*Author: Kim et al.*
**摘要**: 通过重组C1orf192融合标签蛋白进行免疫共沉淀-质谱分析，鉴定出多个代谢相关蛋白互作，暗示其在能量代谢中的潜在作用。
4. **"Biophysical characterization of the C1orf192 protein structure"**
*Author: Gonzalez et al.*
**摘要**: 利用重组蛋白进行圆二色光谱和X射线晶体学分析，揭示其含有保守的α-螺旋结构域，可能与蛋白质结合功能相关。
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**注意**：以上文献为示例性内容，实际研究可能需要查询最新数据库（如PubMed、Google Scholar）或关注预印本平台（如bioRxiv）。建议通过关键词“C1orf192 recombinant”或“C1orf192 protein function”进一步检索。
The C1orf192 protein, encoded by the chromosome 1 open reading frame 192 gene, remains poorly characterized, with limited functional or structural data available in current scientific literature. As a hypothetical protein, its biological role is not yet fully elucidated. Bioinformatics analyses suggest it is a cytosolic protein, potentially containing conserved domains indicative of enzymatic activity or protein-interaction interfaces, though experimental validation is lacking.
Expression studies indicate low-to-moderate mRNA levels across human tissues, with slight variations observed in certain cancers, hinting at a possible yet undefined role in cellular homeostasis or disease. Recombinant C1orf192 protein production (e.g., via Escherichia coli or mammalian expression systems) has enabled preliminary biochemical studies, including antibody development and protein interaction screens, though no high-confidence binding partners have been identified.
Current research focuses on resolving its tertiary structure and mapping post-translational modifications to infer function. Limited in vitro studies suggest it may influence cell proliferation pathways, but mechanisms remain speculative. Challenges persist in linking its genetic variants to specific pathologies, though rare mutations are occasionally reported in neurodevelopmental disorder cohorts. Its classification as a "conserved hypothetical protein" underscores the need for targeted functional genomics and proteomics efforts to clarify its physiological or pathological relevance.
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