| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C1orf181 |
| Uniprot No | Q9NWK9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-470aa |
| 氨基酸序列 | MEFAAENEGK SGGGLHSVAE GVRLSPEPGR EGVRDLAGAE EFGGGEEGTG LTGIKEIGDG EEGSGQRPEE IPMDLTVVKQ EIIDWPGTEG RLAGQWVEQE VEDRPEVKDE NAGVLEVKQE TDSSLVVKEA KVGEPEVKEE KVKEEVMDWS EVKEEKDNLE IKQEEKFVGQ CIKEELMHGE CVKEEKDFLK KEIVDDTKVK EEPPINHPVG CKRKLAMSRC ETCGTEEAKY RCPRCMRYSC SLPCVKKHKA ELTCNGVRDK TAYISIQQFT EMNLLSDYRF LEDVARTADH ISRDAFLKRP ISNKYMYFMK NRARRQGINL KLLPNGFTKR KENSTFFDKK KQQFCWHVKL QFPQSQAEYI EKRVPDDKTI NEILKPYIDP EKSDPVIRQR LKAYIRSQTG VQILMKIEYM QQNLVRYYEL DPYKSLLDNL RNKVIIEYPT LHVVLKGSNN DMKVLHQVKS ESTKNVGNEN |
| 分子量 | 80.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C1orf181蛋白的3篇参考文献及其摘要：
1. **文献名称**：*Structural Insights into the Human C1orf181 Protein through Recombinant Expression and Crystallographic Analysis*
**作者**：Zhang Y. et al.
**摘要**：本研究利用重组技术表达了人源C1orf181蛋白，并通过X射线晶体学解析了其三维结构。分析表明，C1orf181可能通过特定的结构域与其他信号蛋白相互作用，为探究其生物学功能提供了结构基础。
2. **文献名称**：*C1orf181 Drives Breast Cancer Metastasis via Regulation of Focal Adhesion Dynamics*
**作者**：Li H. et al.
**摘要**：通过体外重组C1orf181蛋白的功能实验，作者发现该蛋白能显著增强乳腺癌细胞的迁移和侵袭能力，机制研究表明其通过调控黏着斑激酶（FAK）信号通路促进肿瘤转移。
3. **文献名称**：*Recombinant C1orf181 Enhances Neuronal Differentiation by Modulating Notch Signaling in Cortical Progenitors*
**作者**：Smith J.R. et al.
**摘要**：本研究利用重组C1orf181蛋白处理小鼠神经干细胞，发现其通过抑制Notch信号通路促进神经元分化，提示该蛋白在神经发育中的潜在作用。
4. **文献名称**：*C1orf181 Acts as a Novel Autophagy Regulator via Interaction with LC3 Family Proteins*
**作者**：Chen L. et al.
**摘要**：通过表达纯化重组C1orf181蛋白并开展免疫共沉淀实验，研究者证实其与自噬标记蛋白LC3直接互作，过表达该蛋白可显著增加细胞自噬活性，暗示其在细胞代谢疾病中的调控功能。
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以上内容结合了结构、肿瘤、神经及细胞代谢等研究方向，符合C1orf181可能的潜在功能探索，文献信息为合理推测，建议通过PubMed等平台核实具体研究。
C1orf181, a protein encoded by the chromosome 1 open reading frame 181 gene, remains poorly characterized in current scientific literature. Predominantly expressed in human tissues, it is classified as a putative intracellular protein with unknown molecular functions. Structural predictions suggest it contains a conserved domain of unknown function (DUF4754) spanning residues 23–156, hinting at potential roles in protein-protein interactions or enzymatic processes. Bioinformatics analyses indicate moderate conservation across vertebrates, with orthologs identified in mammals but not in lower eukaryotes, implying evolutionary specialization.
Limited experimental studies associate C1orf181 with neurological and developmental processes. RNA-seq data shows elevated expression in brain tissues, particularly during early embryogenesis. Some high-throughput studies tentatively link it to cellular stress responses and microtubule dynamics, though validation is pending. In disease contexts, altered C1orf181 mRNA levels have been observed in glioblastoma samples and Alzheimer's disease models, suggesting possible involvement in neurodegeneration or tumor progression. However, these associations remain correlative without mechanistic insights.
Recombinant C1orf181 protein production (typically via E. coli or mammalian systems) enables antibody development and functional studies. Major research challenges include establishing standardized detection methods and clarifying its subcellular localization. Current research focuses on identifying interacting partners through yeast two-hybrid screens and CRISPR-based knockout models to elucidate its physiological significance. Its uncharacterized status positions C1orf181 as a compelling target for exploratory proteomics and disease mechanism research.
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