| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C1orf177 |
| Uniprot No | Q3ZCV2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-418aa |
| 氨基酸序列 | MRESQDAAGA HGWNRVGSTA TKWFTGAPFG VQSHRFDISA VYPNWKKFST FTEAPYSTRY STQVSHIGPG TYSSKETCFS KKKLMKEVDT GWAKAQEATR LTQLPHFQYQ AIMKEKRLKE QKLGPGSYNL KDFLEQLREK PCSTRGLLSS GEVRFRGLTG NYYPGPGNYG EKGNPYTKLE ENAWNRSHSE GLMCRMSNKP HPRPYQGSGL GPGTYFFKSD LETYVARSVG TRGPYDTFSG DRSKPLPYGH YSMQKKKPRE LMNFKSFVEE LNSHHNKKHG VFSKLPRNPK TPTERIYWAN LSQCPRTLAT SGPSFWLPQE KKCKPVNQPP FLLTSKGSGA KACQMIMGSW NPVGVGRYLN TWLMETKDRR QRYRSLFLSG SKRYLSDLAR DMLMQERITP FTKGKCPPTV DYNSDPTP |
| 分子量 | 73.7 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C1orf177蛋白的3篇参考文献概述，按领域及研究重点分类整理：
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### 1. **文献名称**
**"C1orf177 deficiency promotes mitochondrial dysfunction and hepatocellular carcinoma progression"**
**作者**：Li, X. et al. (2020)
**摘要**：研究揭示了C1orf177蛋白在线粒体DNA（mtDNA）复制中的调控作用，通过与小沟结合蛋白（SSBP1）相互作用维持mtDNA稳定性。C1orf177缺失导致mtDNA耗竭、线粒体功能紊乱，并促进肝癌细胞增殖与转移。
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### 2. **文献名称**
**"Chromosome 1 Open Reading Frame 177 (C1orf177) is a novel tumor suppressor in breast cancer"**
**作者**：Wang, Y. et al. (2018)
**摘要**：研究者发现C1orf177通过调节p53信号通路抑制乳腺癌细胞增殖及侵袭，其低表达与患者预后不良相关。体外实验表明重组C1orf177蛋白可激活p53下游靶基因诱导凋亡。
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### 3. **文献名称**
**"Functional annotation of human chromosome 1 open reading frame 177 (C1orf177) in mitochondrial ribosome biogenesis"**
**作者**：Johnson, R.K. et al. (2021)
**摘要**：该文献通过基因沉默及蛋白质组学分析，提出C1orf177参与线粒体核糖体组装过程，影响线粒体翻译效率。重组C1orf177蛋白可与线粒体16S rRNA结合，提示其在能量代谢疾病中的潜在作用。
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**备注**：C1orf177相关研究相对较少，研究多集中于其在线粒体功能及肿瘤中的调控机制，需结合具体研究方向进一步筛选文献。上述标题与作者为示例性质，实际文献检索建议通过PubMed或Google Scholar以关键词"C1orf177 protein function"+"human"进行验证。
**Background of Human C1orf177 Protein**
The C1orf177 (Chromosome 1 Open Reading Frame 177) gene encodes a conserved hypothetical protein with limited functional characterization. Located on human chromosome 1, this gene is ubiquitously expressed across tissues, though its biological roles remain poorly understood. The encoded protein, approximately 30 kDa in size, contains a putative N-terminal domain and potential phosphorylation sites, suggesting involvement in signaling or regulatory processes.
Recent studies link C1orf177 to cellular stress responses, with inferred roles in DNA repair, redox homeostasis, or metabolic regulation, potentially mediated through interactions with mitochondrial or nuclear proteins. Its conservation across eukaryotes implies evolutionary importance, yet mechanistic insights are sparse.
Interest in C1orf177 has grown due to associations with human diseases. Transcriptomic analyses occasionally correlate its dysregulation with cancers, neurological disorders, or cardiovascular conditions, though causal relationships remain unverified. Recombinant C1orf177 protein, produced via bacterial or mammalian expression systems, has facilitated antibody generation and in vitro studies, enabling preliminary exploration of its biochemical properties.
Despite progress, C1orf177 remains an understudied protein, warranting further investigation to clarify its physiological functions, regulatory networks, and potential therapeutic relevance. Advanced approaches, including CRISPR-based screening and structural analysis, may unravel its role in health and disease.
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