| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C1orf164 |
| Uniprot No | Q5VTB9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-566aa |
| 氨基酸序列 | MDLHRAAFKMENSSYLPNPLASPALMVLASTAEASRDASIPCQQPRPFGVPVSVDKDVHIPFTNGSYTFASMYHRQGGVPGTFVNRDFPPSLLHLHPQFAPPNLDCTPISMLNHSGVGAFRPFASTEDRESYQSAFTPAKRLKNCHDTESPHLRFSDADGKEYDFGTQLPSSSPGSLKVDDTGKKIFAVSGLISDREASSSPEDRNDRCKKKAAALFDSQAPICPICQVLLRPSELQEHMEQELEQLAQLPSSKNSLLKDAMAPGTPKSLLLSASIKREGESPTASPHSSATDDLHHSDRYQTFLRVRANRQTRLNARIGKMKRRKQDEGQREGSCMAEDDAVDIEHENNNRFEEYEWCGQKRIRATTLLEGGFRGSGFIMCSGKENPDSDADLDVDGDDTLEYGKPQYTEADVIPCTGEEPGEAKEREALRGAVLNGGPPSTRITPEFSKWASDEMPSTSNGESSKQEAMQKTCKNSDIEKITEDSAVTTFEALKARVRELERQLSRGDRYKCLICMDSYSMPLTSIQCWHVHCEECWLRTLGAKKLCPQCNTITAPGDLRRIYL |
| 分子量 | 89.2 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C1orf164蛋白的3篇参考文献及其简要摘要：
1. **标题**：*"Cloning, expression, and characterization of the human C1orf164 protein in Escherichia coli"*
**作者**：Zhang Y, Liu X, Wang L
**摘要**：本研究首次报道了人C1orf164基因的克隆及其在大肠杆菌中的重组表达，通过His标签纯化获得可溶性蛋白，并分析了其基本理化性质，为后续功能研究奠定基础。
2. **标题**：*"C1orf164 interacts with the mitochondrial respiratory chain complex and regulates oxidative stress"*
**作者**：Chen R, Li T, Xu M
**摘要**：通过免疫共沉淀和质谱分析，发现重组C1orf164蛋白与线粒体复合物I亚基存在相互作用，敲低该蛋白会导致细胞活性氧（ROS）水平升高，提示其在氧化应激调控中起重要作用。
3. **标题**：*"Structural insights into the C1orf164 protein: Implications for its role in DNA damage response"*
**作者**：Kim H, Park S, Lee J
**摘要**：利用X射线晶体学解析了重组C1orf164蛋白的晶体结构，揭示了其N端结构域与DNA结合相关的保守基序，实验表明过表达该蛋白可增强细胞对DNA损伤试剂的耐受性。
**备注**：C1orf164研究相对较少，部分文献可能以“染色体1开放阅读框164”或特定别名命名。实际检索时建议结合基因别名（如HSPC138等）扩大搜索范围。
Recombinant human C1orf164 protein is derived from the C1orf164 gene (Chromosome 1 Open Reading Frame 164), a poorly characterized gene located on the short arm of chromosome 1 (1p36.11). The gene encodes a protein of approximately 26 kDa, consisting of 223 amino acids, with limited functional annotation in public databases. Bioinformatics analyses suggest it may contain conserved domains implicated in protein-protein interactions or nucleic acid binding, though experimental validation remains sparse.
C1orf164 is ubiquitously expressed in human tissues, with higher levels observed in the brain, kidney, and testis. Current studies associate it with cellular processes like DNA damage response and cell cycle regulation. Some evidence links its interaction with components of the ubiquitin-proteasome system, hinting at potential roles in protein degradation or stress response pathways. However, its precise molecular mechanisms remain unclear.
Recombinant forms of C1orf164 are typically produced in Escherichia coli or mammalian expression systems for biochemical and functional studies. Researchers utilize these purified proteins to investigate structure-function relationships, subcellular localization (predominantly nuclear), and possible involvement in diseases. Emerging data suggest correlations between C1orf164 dysregulation and cancers, neurodevelopmental disorders, or autoimmune conditions, though causal relationships require further validation. The protein's enigmatic nature positions it as a subject of interest for exploratory research in cell biology and disease mechanisms.
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