| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C1orf135 |
| Uniprot No | Q9H7T9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-357aa |
| 氨基酸序列 | MRRTGPEEEA CGVWLDAAAL KRRKVQTHLI KPGTKMLTLL PGERKANIYF TQRRAPSTGI HQRSIASFFT LQPGKTNGSD QKSVSSHTES QINKESKKNA TQLDHLIPGL AHDCMASPLA TSTTADIQEA GLSPQSLQTS GHHRMKTPFS TELSLLQPDT PDCAGDSHTP LAFSFTEDLE SSCLLDRKEE KGDSARKWEW LHESKKNYQS MEKHTKLPGD KCCQPLGKTK LERKVSAKEN RQAPVLLQTY RESWNGENIE SVKQSRSPVS VFSWDNEKND KDSWSQLFTE DSQGQRVIAH NTRAPFQDVT NNWNWDLGPF PNSPWAQCQE DGPTQNLKPD LLFTQDSEGN QVIRHQF |
| 分子量 | 66.7 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C1orf135蛋白的模拟参考文献示例（注意：实际文献可能存在差异，请以实际数据库检索结果为准）：
1. **文献名称**: *Functional Characterization of C1orf135 as a Novel Tumor Suppressor in Colorectal Cancer*
**作者**: Li X, Wang Y, Zhang Q
**摘要**: 研究揭示了C1orf135在结直肠癌中的表达下调及其通过调控Wnt/β-catenin通路抑制肿瘤细胞增殖的机制。重组蛋白体外实验表明其可诱导细胞周期停滞。
2. **文献名称**: *Structural Insights into the DNA-Binding Activity of Human C1orf135 Protein*
**作者**: Park S, Kim H, Lee J
**摘要**: 通过X射线晶体学解析了C1orf135的DNA结合结构域，发现其与端粒重复序列特异性结合，推测其在基因组稳定性中起关键作用。
3. **文献名称**: *C1orf135 Expression Correlates with Neurodegeneration in Alzheimer's Disease Models*
**作者**: Müller R, Costa A, Schröder J
**摘要**: 在小鼠模型中，重组C1orf135蛋白的过表达减少了β-淀粉样蛋白聚集，并通过激活自噬通路延缓神经元退化。
4. **文献名称**: *Recombinant C1orf135 Protein Production in E. coli: Purification and Functional Analysis*
**作者**: Gupta R, Patel S, Kumar M
**摘要**: 开发了一种高效的重组C1orf135蛋白大肠杆菌表达系统，并通过质谱验证其翻译后修饰，证实其在体外具有抗氧化活性。
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**注意**：以上文献为示例性质，具体内容需通过专业数据库（如PubMed、Web of Science）检索真实文献。若研究领域较新，建议补充关键词组合（如C1orf135 + cancer/metabolism/structure）扩大检索范围。
**Background of Recombinant Human C1orf135 Protein**
The C1orf135 gene, located on chromosome 1 (1p36.11), encodes a protein with limited functional characterization, often categorized as a poorly annotated open reading frame (ORF). The recombinant human C1orf135 protein is produced through genetic engineering for biochemical and cellular studies. Structurally, it is predicted to be a small protein (∼20 kDa) with conserved motifs suggesting potential roles in RNA binding or protein interactions. Bioinformatic analyses indicate low-complexity regions and phosphorylation sites, hinting at regulatory functions. Its subcellular localization remains debated, with studies reporting mitochondrial, nuclear, or cytoplasmic distribution depending on cell type or experimental conditions.
Expression of C1orf135 is tissue-specific, with higher levels observed in testes, thyroid, and kidneys, implying roles in reproductive or metabolic processes. Preliminary studies link it to stress response pathways, cell cycle regulation, and RNA metabolism, though mechanistic insights are lacking. Disease associations are emerging: aberrant C1orf135 expression correlates with cancers (e.g., breast tumors, glioblastoma) and neurodegenerative disorders, potentially via dysregulated apoptosis or genomic instability.
Despite these clues, C1orf135 remains understudied, necessitating functional genomics and proteomic approaches to clarify its physiological and pathological relevance. Recombinant protein tools enable exploration of its interactions, post-translational modifications, and therapeutic potential as a biomarker or drug target.
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