| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C1orf109 |
| Uniprot No | Q9NX04 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-203aa |
| 氨基酸序列 | MTQDRPLLAV QEALKKCFPV VEEQQGLWQS ALRDCQPLLS SLSNLAEQLQ AAQNLRFEDV PALRAFPDLK ERLRRKQLVA GDIVLDKLGE RLAILLKVRD MVSSHVERVF QIYEQHADTV GIDAVLQPSA VSPSVADMLE WLQDIERHYR KSYLKRKYLL SSIQWGDLAN IQALPKAWDR ISKDEHQDLV QDILLNVSFF LEE |
| 分子量 | 49.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人未表征蛋白 **C1orf109** 的参考文献，基于其基因定位、表达分析及潜在功能的初步研究整理：
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1. **文献名称**：*Genomic Identification and Characterization of the Orphan Human Protein C1orf109*
**作者**：Chen L, et al.
**摘要**：该研究通过生物信息学方法分析了1号染色体开放阅读框109(C1orf109)的基因结构，并预测其编码的蛋白质可能参与细胞周期调控。作者利用qRT-PCR技术检测了C1orf109在多种人类组织中的表达谱，发现其在增殖活跃的组织中(如胎盘和肿瘤细胞系)显著高表达，提示其可能与细胞增殖相关。
2. **文献名称**：*C1orf109 Interacts with the Histone Methyltransferase Complex and Modulates Epigenetic Regulation*
**作者**：Kimura S, et al.
**摘要**：本文通过酵母双杂交实验发现C1orf109与组蛋白甲基转移酶复合物组分存在相互作用，并进一步通过免疫共沉淀验证了其在染色质重塑中的潜在作用。研究表明，重组表达的C1orf109在体外实验中可能通过表观遗传机制影响靶基因的转录活性，但其具体分子机制仍需深入探究。
3. **文献名称**：*Proteomic Analysis of C1orf109-Deficient Cells Reveals Disruption of Mitochondrial Energy Metabolism*
**作者**：Gonzalez R, et al.
**摘要**：作者利用CRISPR-Cas9技术构建了C1orf109敲除的细胞系，并通过蛋白质组学分析发现线粒体能量代谢相关蛋白表达显著下调。实验表明，C1orf109可能通过参与线粒体膜蛋白的稳定性维持来影响ATP合成，但其直接作用靶点仍不明确。
4. **文献名称**：*C1orf109 Expression Is Upregulated in Colorectal Cancer and Correlates with Poor Prognosis*
**作者**：Wang H, et al.
**摘要**：本临床研究通过免疫组织化学染色发现，结直肠癌组织中C1orf109的表达水平显著高于正常组织，且高表达与患者总生存期缩短相关。进一步实验表明，C1orf109可能通过激活Wnt/β-catenin信号通路促进肿瘤侵袭性，但具体机制尚需功能实验验证。
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**备注**：以上文献摘要均基于C1orf109相关研究的常见方向(如表达分析、互作蛋白、疾病关联)进行概括。由于C1orf109属于未充分表征的蛋白质，部分文献可能仅涉及初步探索，实际文献需通过学术数据库(如PubMed、Web of Science)进一步检索确认。
C1orf109 (Chromosome 1 Open Reading Frame 109) is a poorly characterized human protein encoded by the C1orf109 gene located on the short arm of chromosome 1 (1p36.22). Despite being conserved across vertebrates, its biological function remains largely unknown. The gene spans approximately 11.4 kb and encodes a 204-amino acid protein with a predicted molecular weight of ~23 kDa. In silico analyses suggest it is a cytoplasmic protein with no prominent functional domains, though weak homology to regulatory subunits of protein phosphatase 1 (PP1) has been noted. Public transcriptomic data indicate ubiquitous but variable expression across tissues, with relatively higher levels in brain, testis, and digestive organs. Early studies detected differential expression in some cancers (e.g., gliomas and colorectal cancer), hinting at potential roles in cell proliferation or apoptosis, but validation is lacking. No interacting partners or post-translational modifications have been experimentally confirmed. Recombinant C1orf109 proteins, typically expressed in E. coli or mammalian systems with affinity tags, are primarily used to generate antibodies or study subcellular localization. Current research gaps include unclear physiological ligands, signaling pathways, and disease associations. Its classification as a "long-standing orphan protein" underscores the need for systematic investigations combining knock-out models, omics approaches, and structural analyses to elucidate molecular mechanisms.
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