| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C10orf3 |
| Uniprot No | Q53EZ4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-464aa |
| 氨基酸序列 | MSSRSTKDLIKSKWGSKPSNSKSETTLEKLKGEIAHLKASVDEITSGKGKLTDKERHRLLEKIRVLEAEKEKNAYQLTEKDKEIQRLRDQLKARYSTTALLEQLEETTREGERREQVLKALSEEKDVLKQQLSAATSRIAELESKTNTLRLSQTVAPNCFNSSINNIHEMEIQLKDALEKNQQWLVYDQQREVYVKGLLAKIFELEKKTETAAHSLPQQTKKPESEGYLQEEKQKCYNDLLASAKKDLEVERQTITQLSFELSEFRRKYEETQKEVHNLNQLLYSQRRADVQHLEDDRHKTEKIQKLREENDIARGKLEEEKKRSEELLSQVQFLYTSLLKQQEEQTRVALLEQQMQACTLDFENEKLDRQHVQHQLLVILKELRKARNQITQLESLKQLHEFAITEPLVTFQGETENREKVAASPKSPTAALNESLVECPKCNIQYPATEHRDLLVHVEYCSK |
| 分子量 | 76.78 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C10orf3(TRAIP)蛋白的模拟参考文献示例(仅供参考，实际文献需通过学术数据库查询)：
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1. **文献名称**: *Structural and functional characterization of human C10orf3 (TRAIP) in DNA damage response*
**作者**: Johnson A., et al.
**摘要**: 本研究通过冷冻电镜解析了C10orf3的三维结构，揭示其通过RING结构域参与泛素化修饰，调控DNA损伤修复通路中的关键分子(如PCNA)，并证明其缺失导致细胞对电离辐射敏感性增加。
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2. **文献名称**: *C10orf3/TRAIP regulates cell cycle progression via p53-dependent signaling*
**作者**: Chen L., et al.
**摘要**: 发现C10orf3通过与p53蛋白互作，调控G1/S期检查点，抑制细胞增殖。在肿瘤细胞中敲低C10orf3会导致基因组不稳定性和加速肿瘤生长，提示其在癌症中的抑癌作用。
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3. **文献名称**: *TRAIP mutations disrupt embryonic development and cause microcephaly in a humanized mouse model*
**作者**: Wang Y., et al.
**摘要**: 构建携带C10orf3突变的小鼠模型，发现其导致神经前体细胞凋亡异常及大脑皮层发育缺陷，与人类小头畸形相关，证实C10orf3对神经发育至关重要。
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4. **文献名称**: *C10orf3 as a potential biomarker for colorectal cancer prognosis*
**作者**: Gupta S., et al.
**摘要**: 通过临床样本分析发现，C10orf3在结直肠癌中表达显著下调，且低表达与患者生存率降低相关，提示其可作为预后标志物及治疗靶点。
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**注**: 以上内容为示例，并非真实文献。实际研究中请通过PubMed、Web of Science等平台查询最新文献。
**Background of Recombinant Human C10orf3 Protein**
The C10orf3 (chromosome 10 open reading frame 3) protein, also known as TDP2 or mitochondrial coiled-coil domain-containing protein 1 (MCCD1), is encoded by the *C10orf3* gene located on human chromosome 10q24.31. Initially identified through genomic sequencing, its biological role has been progressively elucidated. C10orf3 is a multifunctional protein characterized by a conserved phosphodiesterase domain, enabling enzymatic activity in DNA repair by resolving 5'-phosphotyrosine bonds generated by topoisomerase II cleavage—a critical process for maintaining genomic stability.
Beyond DNA repair, C10orf3 localizes to mitochondria and interacts with mitochondrial RNA granules, suggesting roles in mitochondrial RNA metabolism and oxidative phosphorylation. Studies link its dysfunction to neurodegenerative disorders, cancer, and mitochondrial diseases. For example, mutations in *C10orf3* are associated with progressive external ophthalmoplegia (PEO), a mitochondrial disorder marked by impaired mtDNA maintenance.
Recombinant human C10orf3 protein is engineered for functional studies, enabling exploration of its enzymatic mechanisms, interaction partners, and therapeutic potential. Recent research highlights its dual nuclear-mitochondrial localization and involvement in stress responses, positioning it as a potential biomarker or drug target for diseases linked to DNA damage or mitochondrial dysfunction. However, its regulatory pathways and tissue-specific roles remain under investigation, necessitating further studies to fully unravel its biological significance.
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