| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BXDC1 |
| Uniprot No | Q9H7B2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-306aa |
| 氨基酸序列 | MDTLDRVVKPKTKRAKRFLEKREPKLNENIKNAMLIKGGNANATVTKVLKDVYALKKPYGVLYKKKNITRPFEDQTSLEFFSKKSDCSLFMFGSHNKKRPNNLVIGRMYDYHVLDMIELGIENFVSLKDIKNSKCPEGTKPMLIFAGDDFDVTEDYRRLKSLLIDFFRGPTVSNIRLAGLEYVLHFTALNGKIYFRSYKLLLKKSGCRTPRIELEEMGPSLDLVLRRTHLASDDLYKLSMKMPKALKPKKKKNISHDTFGTTYGRIHMQKQDLSKLQTRKMKGLKKRPAERITEDHEKKSKRIKKN |
| 分子量 | 62 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人BXDC1蛋白的3篇参考文献示例(注：文献为假设性描述，实际需根据真实数据库检索)：
1. **文献名称**：*"Functional Analysis of Recombinant Human BXDC1 in DNA Damage Repair"*
**作者**：Chen L, et al.
**摘要**：通过在大肠杆菌中表达重组BXDC1蛋白，研究发现其能够与BRCA1相互作用，在DNA损伤修复通路中促进同源重组修复。
2. **文献名称**：*"Crystal Structure and Biochemical Characterization of Recombinant BXDC1 Protein"*
**作者**：Gupta S, et al.
**摘要**：解析了重组BXDC1的晶体结构，揭示了其泛素结合结构域的保守性，并证明其通过泛素化修饰参与蛋白酶体降解调控。
3. **文献名称**：*"Recombinant BXDC1 Attenuates Oxidative Stress in Neuronal Cells"*
**作者**：Tanaka K, et al.
**摘要**：体外实验表明，重组BXDC1蛋白通过调节Nrf2通路显著减少活性氧(ROS)积累，提示其潜在神经保护功能。
*注：若实际文献有限，建议结合“BXDC1”的别名(如官方基因符号)或相关通路扩展检索。*
The recombination human BXDC1 protein, also known as Ska3 (Spindle and Kinetochore-Associated complex subunit 3), is a critical component of the Ska complex, which plays an essential role in ensuring accurate chromosome segregation during mitosis. This 35 kDa protein, encoded by the BXDC1 gene on chromosome 13. forms a heterotrimeric complex with Ska1 and Ska2. facilitating stable microtubule attachment to kinetochores and proper spindle dynamics. The Ska complex acts as a molecular link between kinetochores and depolymerizing microtubules, coordinating chromosome alignment and anaphase progression.
BXDC1/Ska3 is indispensable for maintaining the metaphase plate and mitotic checkpoint signaling. Structural studies reveal its C-terminal domain mediates complex assembly, while the N-terminal region interacts with microtubules. Dysregulation of BXDC1 is implicated in chromosomal instability, mitotic errors, and cancer progression. Overexpression has been observed in various malignancies, correlating with poor prognosis and chemotherapy resistance.
Recombinant BXDC1 protein, typically produced in bacterial or mammalian expression systems, enables functional studies of kinetochore-microtubule interactions, drug screening, and structural analysis. Its role in checkpoint silencing and Aurora B kinase regulation highlights its potential as a therapeutic target for anticancer therapies targeting mitotic fidelity.
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