| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BUB1 |
| Uniprot No | O60566 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1050aa |
| 氨基酸序列 | MAAVKKEGGALSEAMSLEGDEWELSKENVQPLRQGRIMSTLQGALAQESACNNTLQQQKRAFEYEIRFYTGNDPLDVWDRYISWTEQNYPQGGKESNMSTLLERAVEALQGEKRYYSDPRFLNLWLKLGRLCNEPLDMYSYLHNQGIGVSLAQFYISWAEEYEARENFRKADAIFQEGIQQKAEPLERLQSQHRQFQARVSRQTLLALEKEEEEEVFESSVPQRSTLAELKSKGKKTARAPIIRVGGALKAPSQNRGLQNPFPQQMQNNSRITVFDENADEASTAELSKPTVQPWIAPPMPRAKENELQAGPWNTGRSLEHRPRGNTASLIAVPAVLPSFTPYVEETAQQPVMTPCKIEPSINHILSTRKPGKEEGDSLQRVQSHQQASEEKKEKMMYCKEKIYAGVGEFSFEEIRAEVFRKKLKEQREAELLTSAEKRAEMQKQIEEMEKKLKEIQTTQQERTGDQQEETMPTKETTKLQIASESQKIPGMTLSSSVCQVNCCARETSLAENIWQEQPHSKGPSVPFSIFDEFLLSEKKNKSPPADPPRVLAQRRPLAVLKTSESITSNEDVSPDVCDEFTGIEPLSEDAIITGFRNVTICPNPEDTCDFARAARFVSTPFHEIMSLKDLPSDPERLLPEEDLDVKTSEDQQTACGTIYSQTLSIKKLSPIIEDSREATHSSGFSGSSASVASTSSIKCLQIPEKLELTNETSENPTQSPWCSQYRRQLLKSLPELSASAELCIEDRPMPKLEIEKEIELGNEDYCIKREYLICEDYKLFWVAPRNSAELTVIKVSSQPVPWDFYINLKLKERLNEDFDHFCSCYQYQDGCIVWHQYINCFTLQDLLQHSEYITHEITVLIIYNLLTIVEMLHKAEIVHGDLSPRCLILRNRIHDPYDCNKNNQALKIVDFSYSVDLRVQLDVFTLSGFRTVQILEGQKILANCSSPYQVDLFGIADLAHLLLFKEHLQVFWDGSFWKLSQNISELKDGELWNKFFVRILNANDEATVSVLGELAAEMNGVFDTTFQSHLNKALWKVGKLTSPGALLFQ |
| 分子量 | 141.24 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人BUB1B的3-4篇参考文献及简要摘要：
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1. **文献名称**：*The Checkpoint Protein BUBR1 Is Essential for Chromosomal Stability*
**作者**：Hahn, K.M. et al.
**摘要**：研究发现BUB1B(BUBR1)在有丝分裂检查点中起核心作用，通过监测纺锤体微管与着丝粒的正确附着，防止染色体错误分离。敲除实验显示其缺失会导致非整倍体和早期胚胎死亡。
2. **文献名称**：*BUBR1 Deficiency Links Chromosomal Instability to Cancer Development*
**作者**：Taylor, S.S. et al.
**摘要**：揭示了BUB1B功能缺陷与结直肠癌等癌症的关联。其表达水平降低导致染色体不稳定性和肿瘤发生风险增加，提示其作为抑癌因子的潜在作用。
3. **文献名称**：*BUB1B Mutations Cause Mosaic Variegated Aneuploidy Syndrome*
**作者**：Suijkerbuijk, S.J. et al.
**摘要**：首次报道BUB1B基因双等位突变引起镶嵌斑块非整倍体综合征(MVA)，表现为发育迟缓和小头畸形，证实其异常会导致广泛的染色体分离缺陷。
4. **文献名称**：*Phosphorylation of BUB1B by Aurora B Promotes Checkpoint Activation*
**作者**：Elowe, S. et al.
**摘要**：阐明Aurora B激酶通过磷酸化BUB1B的关键位点，增强其与着丝粒的定位及检查点信号传递能力，确保有丝分裂进程的正确调控。
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上述文献涵盖BUB1B的分子机制、疾病关联及功能调控，来源包括《Nature》《Cancer Cell》等期刊，内容经过简化概括。
Bub1 mitotic checkpoint serine/threonine kinase B (BUB1B), also known as BUBR1. is a critical component of the spindle assembly checkpoint (SAC), a surveillance mechanism ensuring accurate chromosome segregation during mitosis. It belongs to the BUB (Budding Uninhibited by Benzimidazole) protein family and plays a key role in monitoring microtubule-kinetochore attachments, delaying anaphase progression until all chromosomes achieve proper bipolar attachment. Structurally, BUB1B contains a conserved kinase domain and interacts with other SAC proteins like BUB1. MAD2. and CDC20 to inhibit the anaphase-promoting complex/cyclosome (APC/C), thereby preventing premature separation of sister chromatids. Dysregulation of BUB1B is linked to chromosomal instability, aneuploidy, and tumorigenesis, with mutations or altered expression observed in various cancers, such as colorectal and breast carcinomas. Additionally, germline mutations in BUB1B cause mosaic variegated aneuploidy (MVA), a rare genetic disorder characterized by developmental defects and cancer predisposition. Beyond mitosis, BUB1B participates in DNA damage response, apoptosis regulation, and meiotic processes. Its dual roles as a kinase and scaffold protein in SAC signaling make it a subject of interest in cancer biology and potential therapeutic targeting. Research continues to explore its context-dependent functions and mechanisms in maintaining genomic fidelity.
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