| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BUB1B |
| Uniprot No | O60566 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1050aa |
| 氨基酸序列 | MAAVKKEGGALSEAMSLEGDEWELSKENVQPLRQGRIMSTLQGALAQESACNNTLQQQKRAFEYEIRFYTGNDPLDVWDRYISWTEQNYPQGGKESNMSTLLERAVEALQGEKRYYSDPRFLNLWLKLGRLCNEPLDMYSYLHNQGIGVSLAQFYISWAEEYEARENFRKADAIFQEGIQQKAEPLERLQSQHRQFQARVSRQTLLALEKEEEEEVFESSVPQRSTLAELKSKGKKTARAPIIRVGGALKAPSQNRGLQNPFPQQMQNNSRITVFDENADEASTAELSKPTVQPWIAPPMPRAKENELQAGPWNTGRSLEHRPRGNTASLIAVPAVLPSFTPYVEETAQQPVMTPCKIEPSINHILSTRKPGKEEGDSLQRVQSHQQASEEKKEKMMYCKEKIYAGVGEFSFEEIRAEVFRKKLKEQREAELLTSAEKRAEMQKQIEEMEKKLKEIQTTQQERTGDQQEETMPTKETTKLQIASESQKIPGMTLSSSVCQVNCCARETSLAENIWQEQPHSKGPSVPFSIFDEFLLSEKKNKSPPADPPRVLAQRRPLAVLKTSESITSNEDVSPDVCDEFTGIEPLSEDAIITGFRNVTICPNPEDTCDFARAARFVSTPFHEIMSLKDLPSDPERLLPEEDLDVKTSEDQQTACGTIYSQTLSIKKLSPIIEDSREATHSSGFSGSSASVASTSSIKCLQIPEKLELTNETSENPTQSPWCSQYRRQLLKSLPELSASAELCIEDRPMPKLEIEKEIELGNEDYCIKREYLICEDYKLFWVAPRNSAELTVIKVSSQPVPWDFYINLKLKERLNEDFDHFCSCYQYQDGCIVWHQYINCFTLQDLLQHSEYITHEITVLIIYNLLTIVEMLHKAEIVHGDLSPRCLILRNRIHDPYDCNKNNQALKIVDFSYSVDLRVQLDVFTLSGFRTVQILEGQKILANCSSPYQVDLFGIADLAHLLLFKEHLQVFWDGSFWKLSQNISELKDGELWNKFFVRILNANDEATVSVLGELAAEMNGVFDTTFQSHLNKALWKVGKLTSPGALLFQ |
| 分子量 | 141.24 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人有丝分裂检查点丝氨酸/苏氨酸蛋白激酶BUB1β(BUB1B)的文献示例(注：以下内容为模拟摘要，实际文献需通过学术数据库检索确认)：
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1. **文献名称**: *"The human mitotic checkpoint protein BubR1 regulates chromosome-spindle attachments"*
**作者**: Lampson, M. A. & Kapoor, T. M.
**摘要**: 研究揭示BUB1B(又称BubR1)在有丝分裂检查点中的双重作用：不仅参与监测染色体与纺锤体微管的正确连接，还通过调控动力蛋白(kinetochore)复合物确保染色体排列正确，防止错误分离。
2. **文献名称**: *"BUB1B-CDC20 interaction ensures accurate chromosome segregation by blocking APC/C activation"*
**作者**: Suijkerbuijk, S. J. et al.
**摘要**: 发现BUB1B通过磷酸化CDC20并抑制后期促进复合体(APC/C)的活性，从而延缓有丝分裂进程直至所有染色体正确附着于纺锤体，防止非整倍体形成。
3. **文献名称**: *"BUB1B overexpression promotes genomic instability in cancer"*
**作者**: Ricke, R. M. et al.
**摘要**: 在结直肠癌模型中，BUB1B的异常高表达会削弱有丝分裂检查点功能，导致染色体错误分离和基因组异倍化，加速肿瘤进展。
4. **文献名称**: *"BubR1 insufficiency causes premature aging and neurodegeneration in mice"*
**作者**: Baker, D. J. et al.
**摘要**: 研究表明，BUB1B缺陷的小鼠因基因组不稳定性增强，表现出早衰表型(如肌肉萎缩、白内障)，提示其在维持组织稳态和延缓衰老中的关键作用。
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如需具体文献，建议通过 **PubMed** 或 **Google Scholar** 检索关键词(如“BUB1B function”“BubR1 cancer”等)获取最新研究。
Bub1 mitotic checkpoint serine/threonine kinase B (BUB1B), also known as BUBR1. is a critical component of the spindle assembly checkpoint (SAC), a surveillance mechanism that ensures accurate chromosome segregation during mitosis. It plays a pivotal role in monitoring kinetochore-microtubule attachments and delaying anaphase onset until all chromosomes achieve proper bipolar attachment, thereby safeguarding genomic stability. Structurally, BUB1B contains conserved kinase and KEN box domains, enabling interactions with other checkpoint proteins like BUB3 and CDC20. Its kinase activity contributes to SAC signaling by phosphorylating downstream targets, while its scaffolding function stabilizes the mitotic checkpoint complex. Dysregulation of BUB1B is linked to chromosomal instability, aneuploidy, and tumorigenesis. Germline mutations in BUB1B are associated with mosaic variegated aneuploidy syndrome, a rare disorder characterized by developmental defects and cancer predisposition. Conversely, BUB1B overexpression has been observed in various cancers, correlating with poor prognosis. Recent studies also highlight its non-canonical roles in DNA damage response, aging, and immune regulation. As a dual-function protein balancing kinase-dependent and kinase-independent activities, BUB1B represents a potential therapeutic target for cancer and chromosomal instability disorders, though its complex signaling network requires further elucidation.
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