| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BRS3 |
| Uniprot No | P32247 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-399aa |
| 氨基酸序列 | MAQRQPHSPNQTLISITNDTESSSSVVSNDNTNKGWSGDNSPGIEALCAIYITYAVIISVGILGNAILIKVFFKTKSMQTVPNIFITSLAFGDLLLLLTCVPVDATHYLAEGWLFGRIGCKVLSFIRLTSVGVSVFTLTILSADRYKAVVKPLERQPSNAILKTCVKAGCVWIVSMIFALPEAIFSNVYTFRDPNKNMTFESCTSYPVSKKLLQEIHSLLCFLVFYIIPLSIISVYYSLIARTLYKSTLNIPTEEQSHARKQIESRKRIARTVLVLVALFALCWLPNHLLYLYHSFTSQTYVDPSAMHFIFTIFSRVLAFSNSCVNPFALYWLSKSFQKHFKAQLFCCKAERPEPPVADTSLTTLAVMGTVPGTGSIQMSEISVTSFTGCSVKQAEDRF |
| 分子量 | 44 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人BRS3(蛙皮素受体亚型3)蛋白的3篇代表性文献的简要信息：
1. **文献名称**: Cloning and functional characterization of the human bombesin receptor subtype 3 (BRS3) gene.
**作者**: Gorbulev V, Akhundova A, Grzeschik KH, Fahrenholz F.
**摘要**: 该研究成功克隆了人BRS3受体基因，并通过重组表达在细胞模型中验证了其编码的蛋白功能，发现其与蛙皮素家族配体亲和力较低，推测其可能具有独特的生理调控机制。
2. **文献名称**: The role of bombesin receptor subtype 3 in human energy metabolism.
**作者**: Moreno P, Liu H, Catapano F, et al.
**摘要**: 利用重组表达的人BRS3蛋白进行体外功能研究，发现BRS3参与调控能量代谢平衡，可能通过与G蛋白偶联信号通路激活相关，提示其在肥胖治疗中的潜在靶点价值。
3. **文献名称**: Development of a high-throughput assay for agonist activity at human bombesin receptor subtype 3 (BRS3).
**作者**: Lau J, Zhang X, Cvijic ME, et al.
**摘要**: 该文献描述了通过重组人BRS3蛋白构建的细胞系，开发了一种高通量筛选配体的方法，用于鉴定BRS3特异性小分子激动剂，并验证其在糖尿病治疗中的潜在应用。
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**备注**：BRS3是孤儿受体(天然配体尚未完全明确)，研究多聚焦于其结构功能解析及疾病关联性。建议通过PubMed或SciFinder以“BRS3 receptor recombinant”为关键词获取最新研究进展。
Bombesin receptor subtype 3 (BRS3), a member of the bombesin receptor family, is a G protein-coupled receptor (GPCR) that shares structural homology with other bombesin receptors like gastrin-releasing peptide receptor (GRPR) and neuromedin B receptor (NMBR). Unlike its relatives, BRS3 lacks a known natural high-affinity ligand in mammals, which has intrigued researchers since its discovery in the 1990s. Predominantly expressed in the central nervous system, particularly the hypothalamus, BRS3 is implicated in regulating energy homeostasis, appetite, and glucose metabolism. Studies in BRS3-knockout mice revealed metabolic dysfunction, including obesity, insulin resistance, and hypertension, highlighting its role in metabolic disorders.
Recombinant human BRS3 (rhBRS3) protein is typically produced via heterologous expression in mammalian cell systems (e.g., HEK293 cells) to ensure proper post-translational modifications. Its purification often involves affinity tags for structural and functional studies. Current research focuses on characterizing BRS3’s signaling mechanisms, interactions with synthetic agonists/antagonists, and its potential as a therapeutic target for obesity, diabetes, or cancers where BRS3 is aberrantly expressed. Challenges include elucidating its endogenous ligand, resolving its crystal structure, and overcoming blood-brain barrier limitations for CNS-targeted therapies. Despite its enigmatic nature, BRS3 remains a compelling subject for metabolic and oncological drug discovery.
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