| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BRRN1 |
| Uniprot No | Q15003 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-741aa |
| 氨基酸序列 | MGPPGPALPA TMNNSSSETR GHPHSASSPS ERVFPMPLPR KAPLNIPGTP VLEDFPQNDD EKERLQRRRS RVFDLQFSTD SPRLLASPSS RSIDISATIP KFTNTQITEH YSTCIKLSTE NKITTKNAFG LHLIDFMSEI LKQKDTEPTN FKVAAGTLDA STKIYAVRVD AVHADVYRVL GGLGKDAPSL EEVEGHVADG SATEMGTTKK AVKPKKKHLH RTIEQNINNL NVSEADRKCE IDPMFQKTAA SFDECSTAGV FLSTLHCQDY RSELLFPSDV QTLSTGEPLE LPELGCVEMT DLKAPLQQCA EDRQICPSLA GFQFTQWDSE THNESVSALV DKFKKNDQVF DINAEVDESD CGDFPDGSLG DDFDANDEPD HTAVGDHEEF RSWKEPCQVQ SCQEEMISLG DGDIRTMCPL LSMKPGEYSY FSPRTMSMWA GPDHWRFRPR RKQDAPSQSE NKKKSTKKDF EIDFEDDIDF DVYFRKTKAA TILTKSTLEN QNWRATTLPT DFNYNVDTLV QLHLKPGTRL LKMAQGHRVE TEHYEEIEDY DYNNPNDTSN FCPGLQAADS DDEDLDDLFV GPVGNSDLSP YPCHPPKTAQ QNGDTPEAQG LDITTYGESN LVAEPQKVNK IEIHYAKTAK KMDMKKLKQS MWSLLTALSG KEADAEANHR EAGKEAALAE VADEKMLSGL TKDLQRSLPP VMAQNLSIPL AFACLLHLAN EKNLKLEGTE DLSDVLVRQG D |
| 分子量 | 82.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人BRRN1(BARR1)蛋白的3篇假设参考文献示例(仅供格式参考)：
1. **文献名称**: *BRRN1 modulates chromatin structure through interaction with SMC complexes during mitosis*
**作者**: Thompson, L. et al.
**摘要**: 该研究发现BRRN1蛋白通过与染色体重构复合物SMC(Structural Maintenance of Chromosomes)相互作用，在有丝分裂中调控染色体凝缩过程，确保基因组稳定性。
2. **文献名称**: *BRRN1 is essential for sister chromatid cohesion via recruiting cohesin loader proteins*
**作者**: Zhang, Y. & Watanabe, K.
**摘要**: 研究证明BRRN1通过募集cohesin复合体加载蛋白(如NIPBL)，维持姐妹染色单体的黏连，缺失BRRN1会导致染色体分离异常及细胞周期停滞。
3. **文献名称**: *Epigenetic regulation by BRRN1: A role in neuronal differentiation*
**作者**: Gomez, M. et al.
**摘要**: 本文揭示BRRN1通过调控组蛋白乙酰化和染色质重塑，影响神经干细胞的基因表达谱，从而促进神经元分化和突触形成。
**备注**：BRRN1在部分文献中也被称为BARR1或Bromodomain Adjacent to Zinc Finger Domain 1B (BAZ1B)，实际文献需根据基因名称或功能关键词(如“chromosome condensation”或“cohesin”)在PubMed/Google Scholar中检索获取。
BRRN1 (Barren homolog 1), also known as NCAP-H, is a key subunit of the condensin I complex, essential for chromosome organization and segregation during mitosis and meiosis. It belongs to the structural maintenance of chromosomes (SMC) protein family, which governs higher-order chromatin architecture. The human BRRN1 gene is located on chromosome 14q11.2 and encodes a 1.325-amino-acid protein containing conserved domains for ATP binding and protein interactions.
Condensin I, comprising BRRN1. SMC2. SMC4. NCAP-D2. and NCAP-G, drives chromosome condensation by inducing positive supercoiling in DNA, ensuring proper sister chromatid resolution. BRRN1 stabilizes the complex and recruits it to chromosomes during prophase. Studies show its critical role in mitotic progression, DNA repair, and transcriptional regulation. Dysregulation of BRRN1 is linked to genomic instability, aneuploidy, and carcinogenesis, with overexpression observed in multiple cancers, correlating with poor prognosis.
Recombinant human BRRN1 protein is produced in vitro (e.g., E. coli or mammalian systems) for functional studies, structural analyses, and drug screening. Its study enhances understanding of cell division mechanisms and potential therapeutic targeting in proliferative disorders.
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