| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BRPF1 |
| Uniprot No | P55201 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1214aa |
| 氨基酸序列 | MGVDFDVKTFCHNLRATKPPYECPVETCRKVYKSYSGIEYHLYHYDHDNPPPPQQTPLRKHKKKGRQSRPANKQSPSPSEVSQSPGREVMSYAQAQRMVEVDLHGRVHRISIFDNLDVVSEDEEAPEEAPENGSNKENTETPAATPKSGKHKNKEKRKDSNHHHHHNVSASTTPKLPEVVYRELEQDTPDAPPRPTSYYRYIEKSAEELDEEVEYDMDEEDYIWLDIMNERRKTEGVSPIPQEIFEYLMDRLEKESYFESHNKGDPNALVDEDAVCCICNDGECQNSNVILFCDMCNLAVHQECYGVPYIPEGQWLCRRCLQSPSRAVDCALCPNKGGAFKQTDDGRWAHVVCALWIPEVCFANTVFLEPIDSIEHIPPARWKLTCYICKQRGSGACIQCHKANCYTAFHVTCAQQAGLYMKMEPVRETGANGTSFSVRKTAYCDIHTPPGSARRLPALSHSEGEEDEDEEEDEGKGWSSEKVKKAKAKSRIKMKKARKILAEKRAAAPVVSVPCIPPHRLSKITNRLTIQRKSQFMQRLHSYWTLKRQSRNGVPLLRRLQTHLQSQRNCDQVGRDSEDKNWALKEQLKSWQRLRHDLERARLLVELIRKREKLKRETIKVQQIAMEMQLTPFLILLRKTLEQLQEKDTGNIFSEPVPLSEVPDYLDHIKKPMDFFTMKQNLEAYRYLNFDDFEEDFNLIVSNCLKYNAKDTIFYRAAVRLREQGGAVLRQARRQAEKMGIDFETGMHIPHSLAGDEATHHTEDAAEEERLVLLENQKHLPVEEQLKLLLERLDEVNASKQSVGRSRRAKMIKKEMTALRRKLAHQRETGRDGPERHGPSSRGSLTPHPAACDKDGQTDSAAEESSSQETSKGLGPNMSSTPAHEVGRRTSVLFSKKNPKTAGPPKRPGRPPKNRESQMTPSHGGSPVGPPQLPIMSSLRQRKRGRSPRPSSSSDSDSDKSTEDPPMDLPANGFSGGNQPVKKSFLVYRNDCSLPRSSSDSESSSSSSSSAASDRTSTTPSKQGRGKPSFSRGTFPEDSSEDTSGTENEAYSVGTGRGVGHSMVRKSLGRGAGWLSEDEDSPLDALDLVWAKCRGYPSYPALIIDPKMPREGMFHHGVPIPVPPLEVLKLGEQMTQEAREHLYLVLFFDNKRTWQWLPRTKLVPLGVNQDLDKEKMLEGRKSNIRKSVQIAYHRALQHRSKVQGEQSSETSDSD |
| 分子量 | 137 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人BRPF1蛋白的参考文献概览(示例为虚构研究内容，仅作参考)：
1. **"BRPF1 is a regulatory subunit of the MOZ histone acetyltransferase complex"**
*作者：Doyon, Y., et al.*
**摘要**：本研究通过重组表达人BRPF1蛋白，揭示了其作为MOZ乙酰转移酶复合体的关键调控亚基，调控组蛋白H3的乙酰化活性，并参与胚胎干细胞的表观遗传重编程。
2. **"Structural basis of BRPF1 activation in the MOF-MSL complex"**
*作者：Ullah, M., et al.*
**摘要**：利用X射线晶体学解析了BRPF1与MOF组蛋白乙酰转移酶的复合物结构，发现BRPF1通过PHD锌指结构域结合组蛋白H3K4me3.调控靶基因转录活性。
3. **"BRPF1 mutations drive epigenetic dysregulation in leukemia"**
*作者：Qiu, Y., et al.*
**摘要**：通过体外重组BRPF1功能域分析，证实其突变(如R415C)破坏与组蛋白H3的结合能力，导致白血病细胞中H3K23乙酰化异常和分化阻滞。
4. **"BRPF1 recruits HP1α to promote heterochromatin formation"**
*作者：Lalonde, M.-E., et al.*
**摘要**：研究发现重组BRPF1蛋白通过结合HP1α蛋白，介导异染色质区组蛋白修饰调控，影响基因组稳定性及细胞周期进程。
(注：以上文献内容为示例，实际研究中请参考具体发表的论文。)
BRPF1 (Bromodomain and PHD Finger-containing Protein 1) is a multidomain scaffold protein critical for assembling and stabilizing the MOZ/MORF histone acetyltransferase (HAT) complexes, which play essential roles in epigenetic regulation. These complexes catalyze acetylation of histone H3 at lysine 23 (H3K23ac), modulating chromatin structure and transcriptional activation. Human BRPF1 contains several functional domains, including a plant homeodomain (PHD), a bromodomain, and a PHD-BROMO adjacent domain (PBAD), enabling interactions with histones, non-histone proteins, and other epigenetic modifiers. It acts as a master regulator by bridging the catalytic MOZ/MORF subunit with other components like ING5 and EAF6. ensuring proper complex assembly and substrate specificity.
BRPF1 is widely expressed in tissues and implicated in embryonic development, neurogenesis, and hematopoiesis. Dysregulation of BRPF1 is linked to developmental disorders and cancers. For instance, germline mutations are associated with intellectual disability and craniofacial anomalies, while somatic alterations contribute to leukemia and solid tumors. Recombinant BRPF1 proteins, typically produced in Escherichia coli or mammalian expression systems with affinity tags (e.g., GST, His-tag), are used to study its structure-function relationships, HAT complex reconstitution, and inhibitor screening. Research on BRPF1 highlights its therapeutic potential as a target for epigenetic drugs, particularly in malignancies driven by aberrant histone acetylation.
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