| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BRI3BP |
| Uniprot No | Q8WY22 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-251aa |
| 氨基酸序列 | MGARASGGPLARAGLLLLLLLLLLLGLLAPGAQGARGRGGAEKNSYRRTVNTFSQSVSSLFGEDNVRAAQKFLARLTERFVLGVDMFVETLWKVWTELLDVLGLDVSNLSQYFSPASVSSSPARALLLVGVVLLAYWFLSLTLGFTFSVLHVVFGRFFWIVRVVLFSMSCVYILHKYEGEPENAVLPLCFVVAVYFMTGPMGFYWRSSPSGPSNPSNPSVEEKLEHLEKQVRLLNIRLNRVLESLDRSKDK |
| 分子量 | 54.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人BRI3结合蛋白(BRI3BP)的模拟参考文献，涵盖其功能、机制及疾病关联研究：
---
1. **文献名称**：*"BRI3BP regulates APP processing by modulating β-secretase activity in Alzheimer's disease models"*
**作者**：Chen L. et al.
**摘要**：研究发现BRI3BP通过直接结合淀粉样前体蛋白(APP)，抑制β-分泌酶(BACE1)的活性，减少β-淀粉样蛋白(Aβ)的产生，提示其在阿尔茨海默病病理中的保护作用。
2. **文献名称**：*"Neuroprotective role of BRI3BP via PI3K/Akt signaling in oxidative stress-induced neuronal apoptosis"*
**作者**：Tanaka K. et al.
**摘要**：该文证实BRI3BP通过激活PI3K/Akt信号通路，抑制氧化应激诱导的神经元凋亡，为神经退行性疾病的治疗提供了潜在靶点。
3. **文献名称**：*"Dysregulation of BRI3BP expression correlates with neuroinflammation in traumatic brain injury"*
**作者**：Wang Y. & Martinez-Lopez M.
**摘要**：在创伤性脑损伤模型中，BRI3BP表达显著下调，其缺失加剧小胶质细胞活化和促炎因子释放，表明其参与调控神经炎症反应。
4. **文献名称**：*"Crystallographic analysis of human BRI3BP reveals a novel binding interface for protein-protein interactions"*
**作者**：Gupta R. et al.
**摘要**：通过解析BRI3BP的晶体结构，发现其C端结构域存在独特的疏水性结合口袋，可能介导与APP、Tau等神经相关蛋白的相互作用。
---
*注：以上文献为模拟内容，实际研究中请查询真实数据库(如PubMed)获取准确信息。*
BRI3-binding protein (BRI3BP), also termed BRI3B or E3 ubiquitin-protein ligase BRI3BP, is a multifunctional protein identified in 2004 as an interaction partner of brain protein BRI3. which is linked to neurodegenerative disorders. Structurally, BRI3BP contains a conserved RING-H2 domain, enabling its role as an E3 ubiquitin ligase in the ubiquitin-proteasome system, which regulates protein degradation, trafficking, and signaling. It is ubiquitously expressed, with higher levels in the brain, heart, and testis. Functionally, BRI3BP modulates apoptosis, autophagy, and neuronal survival through interactions with proteins like BRI3 and amyloid precursor protein (APP). Studies implicate BRI3BP in Alzheimer’s disease pathogenesis, potentially influencing APP processing and amyloid-beta production. It also shows dual roles in cancer, acting as a tumor suppressor in lung and liver cancers by promoting apoptosis, while exhibiting oncogenic properties in certain contexts. BRI3BP’s regulatory effects on ERK and NF-κB pathways further highlight its involvement in stress responses and inflammation. Despite progress, its precise molecular mechanisms, tissue-specific functions, and therapeutic potential remain under investigation, particularly in neurodegeneration and cancer progression. Research continues to explore its interplay with prion-like proteins and disease-related aggregates.
×
