关键信息
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基因名
MRPL4
- 应用
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别名
Large ribosomal subunit protein uL4m. 39S ribosomal protein L4. mitochondrial. L4mt. MRP-L4
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9BYD3
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表达区间
1-263 aa
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氨基酸序列
MLQFVRAGARAWLRPTGSQGLSSLAEEAARATENPEQVASEGLPEPVLRKVELPVPTHRRPVQAWVESLRGFEQERVGLADLHPDVFATAPRLDILHQVAMWQKNFKRISYAKTKTRAEVRGGGRKPWPQKGTGRARHGSIRSPLWRGGGVAHGPRGPTSYYYMLPMKVRALGLKVALTVKLAQDDLHIMDSLELPTGDPQYLTELAHYRRWGDSVLLVDLTHEEMPQSIVEATSRLKTFNLIPAVGEQRAQAPRVRMCRLRC
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分子量
54.67 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
MRPL4, or Mitochondrial Ribosomal Protein L4, is a crucial component of the mitochondrial ribosome, playing a significant role in the protein synthesis machinery within mitochondria. Mitochondria are essential organelles responsible for energy production, and their function is tightly linked to cellular metabolism, growth, and apoptosis. Mutations or dysregulation of MRPL4 have been implicated in various mitochondrial diseases and metabolic disorders, highlighting its importance in human health. Recent studies have focused on characterizing the structural and functional properties of MRPL4 to understand its precise role in mitochondrial translation and its impact on mitochondrial function. The exploration of MRPL4 as a recombinant protein is of particular interest, as it offers potential insights into mitochondrial biogenesis and pathology. Advanced techniques, including cryo-electron microscopy and mass spectrometry, are being employed to elucidate the interactions between MRPL4 and mitochondrial RNA, as well as its interaction with other mitochondrial ribosomal proteins. Furthermore, understanding MRPL4's role in the context of mitochondrial dynamics and its involvement in oxidative stress responses can pave the way for developing therapeutic strategies for mitochondrial-related diseases. This research underscores the significance of MRPL4 not only as a fundamental molecular player in mitochondrial biology but also as a potential target for addressing mitochondrial dysfunctions that contribute to a range of human disorders.
