| 纯度 | >95% |
| 种属 | Human |
| 靶点 | Semaphorin 5B |
| Uniprot No | Q9P283-1 |
| 内毒素 | ＜0.1EU/ug |
| 表达宿主 | 293F |
| 表达区间 | 78-834 aa |
| 活性数据 | Measured by the ability of the immobilized protein to support the adhesion of human pancreatic cancer cells. When 5 x 10^4 cells/well are added to Recombinant Human Semaphorin 5B Fc Chimera coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour at 37 ℃. The ED50 for this effect is 0.7‑2.8 μg/mL. |
| 分子量 | 130- 140 kDa |
| 蛋白标签 | C-hFc&His tag |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Recombinant Human Semaphorin 5B Protein (Active) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than >95%
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