关键信息
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基因名
MRGPRF
- 应用
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别名
MRGPRF; GPR140; GPR168; MRGF; PSEC0142; Mas-related G-protein coupled receptor member F; Mas-related gene F protein; G-protein coupled receptor 140; G-protein coupled receptor 168
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q96AM1
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表达区间
1-343 aa
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氨基酸序列
MAGNCSWEAHPGNRNKMCPGLSEAPELYSRGFLTIEQIAMLPPPAVMNYIFLLLCLCGLV GNGLVLWFFGFSIKRNPFSIYFLHLASADVGYLFSKAVFSILNTGGFLGTFADYIRSVCR VLGLCMFLTGVSLLPAVSAERCASVIFPAWYWRRRPKRLSAVVCALLWVLSLLVTCLHNY FCVFLGRGAPGAACRHMDIFLGILLFLLCCPLMVLPCLALILHVECRARRRQRSAKLNHV ILAMVSVFLVSSIYLGIDWFLFWVFQIPAPFPEYVTDLCICINSSAKPIVYFLAGRDKSQ RLWEPLRVVFQRALRDGAELGEAGGSTPNTVTMEMQCPPGNAS
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分子量
38.1 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
MRGPRF (Mas-related G protein-coupled receptor F) is a member of the Mas-related GPCR family, which is primarily expressed in sensory neurons and plays a significant role in pain perception and inflammatory responses. Research on MRGPRF has gained traction due to its involvement in nociceptive pathways and its potential link to chronic pain conditions and itch sensation. Unlike classical GPCRs, MRGPRF is activated by a variety of endogenous and exogenous ligands, including neuropeptides and certain drugs, which makes it a unique target for therapeutic interventions. Increased expression of MRGPRF has been observed in models of inflammation and neuropathic pain, suggesting it may contribute to the sensitization of sensory neurons in these conditions. Understanding the molecular mechanisms underlying MRGPRF activation and signaling could pave the way for the development of novel analgesics and antipruritic agents. Consequently, the investigation of MRGPRF’s functions, signaling pathways, and interactions with ligands is crucial for elucidating its role in pain and itch modulation, providing insights that may lead to innovative treatments for related disorders. As the research continues to evolve, MRGPRF is becoming a promising target in the field of pain management, and efforts to characterize its biological functions at the molecular level are essential for harnessing its therapeutic potential.
