| 纯度 | >95% |
| 种属 | Human |
| 靶点 | Hip |
| Uniprot No | Q96QV1 |
| 内毒素 | ＜0.1EU/ug |
| 表达宿主 | 293F |
| 表达区间 | 18- 670 aa |
| 活性数据 | Measured by its ability to inhibit Sonic Hedgehog (Shh) induction of alkaline phosphatase production in C3H10T1/2 mouse embryonic fibroblast cells. Recombinant Human Hip inhibits a constant dose of 20 ng/mL of Recombinant Human Sonic Hedgehog/Shh Protein, High Activity in a C3H10T1/2 mouse embryonic fibroblast cell alkaline phosphatase induction assay with an ED50 = 0.1-0.6 µg/mL. |
| 分子量 | 77- 89 kDa |
| 蛋白标签 | C-His |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Recombinant Human Hip Protein (Active) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than >95%
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