| 纯度 | >= 81 % SDS-PAGE |
| 种属 | Human |
| 靶点 | E6AP/UBE3A |
| 表达区间 | KRAAAKHLIERYYHQLTEGCGNEACTNEFCASCPTFLRMDNNAAAIKALE LYKINAKLCDPHPSKKGASSAYLENSKGAPNNSCSEIKMNKKGARIDFKD VTYLTEEKVYEILELCREREDYSPLIRVIGRVFSSAEALVQSFRKVKQHT KEELKSLQAKDEDKDEDEKEKAACSAAAMEEDSEASSSRIGDSSQGDNNL QKLGPDDVSVDIDAIRRVYTRLLSNEKIETAFLNALVYLSPNVECDLTYH NVYSRDPNYLNLFIIVMENRNLHSPEYLEMALPLFCKAMSKLPLAAQGKL IRLWSKYNADQIRRMMETFQQLITYKVISNEFNSRNLVNDDDAIVAASKC LKMVYYANVVGGEVDTNHNEEDDEEPIPESSELTLQELLGEERRNKKGPR VDPLETELGVKTLDCRKPLIPFEEFINEPLNEVLEMDKDYTFFKVETENK FSFMTCPFILNAVTKNLGLYYDNRIRMYSERRITVLYSLVQGQQLNPYLR LKVRRDHIIDDALVRLEMIAMENPADLKKQLYVEFEGEQGVDEGGVSKEF FQLVVEEIFNPDIGMFTYDESTKLFWFNPSSFETEGQFTLIGIVLGLAIY NNCILDVHFPMVVYRKLMGKKGTFRDLGDSHPVLYQSLKDLLEYEGNVED DMMITFQISQTDLFGNPMMYDLKENGDKIPITNENRKEFVNLYSDYILNK SVEKQFKAFRRGFHMVTNESPLKYLFRPEEIELLICGSRNLDFQALEETT EYDGGYTRDSVLIREFWEIVHSFTDEQKRLFLQFTTGTDRAPVGGLGKLK MIIAKNGPDTERLPTSHTCFNVLLLPEYSSKEKLKERLLKAITYAKGFGM L |
| 活性数据 | Assay Conditions: 50 µl of reaction mixture 4 mM ATP, 5 mM MgCl2, 1 mM DTT, E1 (0.5 µg), E2 (2.8 µg), Biotinylated Ubiquitin (1 µg) in 50 mM Tris pH 7.4 was incubated for 30 min at 37°C. Transfer the reaction mixture to a streptavidin-coated plate and incubate for 30 min at 37°C. Add antibody against UBE3A, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminescence. |
| 分子量 | 100 kDa including tags |
| 蛋白标签 | His-DDDDK tag N-Terminus |
| 缓冲液 | Liquid |
| 稳定性 & 储存条件 | Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle. pH: 8.00 This product is an active protein and may elicit a biological response in vivo, handle with caution. |
×
