关键信息
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基因名
MICAL3
- 应用
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别名
MICAL3; KIAA0819; KIAA1364; [F-actin]-monooxygenase MICAL3; EC 1.14.13.225; Molecule interacting with CasL protein 3; MICAL-3
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q7RTP6
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表达区间
1-948 aa
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氨基酸序列
MEERKHETMNPAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLIQPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNINVNFLRPSQVRHLYDTGETKDIHLEMESLVNSRTTPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLPSSDTLDLNAEEKAVLIASTRSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEEAPRGHRGERPTLVSTLTDRRMDVAVGNQNKVKYMATQLLAKFEENAPAQSIGIRRQGSMKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPHYCYRLSGYAQRKRPAVAPLSGKEAKGPLQDGATTDANGRANAVASSTERTPGSGVNGLEEPSIAKRLRGTPERIELENYRLSLRQAEALQEVPEETQAEHNLSSVLDTGAEEDVASRSARRAAGRPPATRP
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分子量
130.68 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
MICAL3 (Molecule Interacting with CasL 3) is a member of the MICAL family of proteins, which are important in cellular processes such as cytoskeleton organization, cell signaling, and apoptosis. The study of MICAL3 has gained interest due to its role in neurodevelopment and its potential implications in neurodegenerative diseases. Research indicates that MICAL3 is involved in the regulation of actin dynamics, particularly through its ability to catalyze the oxidation of actin, leading to the polymerization of filamentous actin (F-actin). Furthermore, MICAL3 has been implicated in various cellular functions, including cell migration, neuronal morphogenesis, and synaptic plasticity. Understanding the structure and function of recombinant MICAL3 has significant implications for elucidating its biological roles and for potential therapeutic interventions in related pathologies. By producing and characterizing recombinant MICAL3, researchers aim to uncover its molecular mechanisms of action and how it interacts with other cellular components, which could illuminate new avenues for tackling diseases associated with cytoskeletal dysregulation. The recombinant form of MICAL3 provides a valuable tool for biophysical and biochemical studies, enabling the exploration of its functionality in a controlled environment. Overall, the investigation of MICAL3 not only enhances our comprehension of fundamental cellular processes but also provides insights into the molecular basis of various diseases, reinforcing its importance in biomedical research.
