关键信息
-
基因名
MED9
- 应用
-
别名
MED9; MED25Mediator of RNA polymerase II transcription subunit 9; Mediator complex subunit 9
-
种属
Human
-
表达系统
E. coli
-
标签
GST-tag at N-terminal
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
Q9NWA0
-
表达区间
2-146aa
-
氨基酸序列
ASAGVAAGR QAEDVLPPTS DQPLPDTKPL PPPQPPPVPA PQPQQSPAPR PQSPARAREE ENYSFLPLVH NIIKCMDKDS PEVHQDLNAL KSKFQEMRKL ISTMPGIHLS PEQQQQQLQS LREQVRTKNE LLQKYKSLCM FEIPKE
-
分子量
16,4 KDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
MED9, a subunit of the Mediator complex, plays a critical role in the regulation of gene transcription by serving as a bridge between transcription factors and RNA polymerase II. The Mediator complex is essential for conveying regulatory signals from gene-specific activators and repressors, thereby modulating the transcription of numerous genes involved in cell growth, differentiation, and response to external stimuli. Recent studies have highlighted the significance of MED9 in various biological processes, including cellular proliferation and apoptosis, and its involvement in cancer progression, making it a target of interest for therapeutic interventions. Additionally, MED9 has been found to interact with several oncogenic proteins, further implicating its role in tumor biology. The recombinant expression of MED9 protein allows for the elucidation of its functional mechanisms and interactions at a molecular level, providing insights into its contributions to gene regulation and potential applications in cancer treatment. Understanding the structure and function of MED9 through recombinant protein studies could pave the way for novel therapeutic strategies by targeting the Mediator complex in diseases characterized by aberrant gene expression. This burgeoning area of research underscores the importance of MED9 not only in transcriptional regulation but also in the development of targeted therapies that could influence lifelong health outcomes.
