关键信息
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基因名
ADRb1
- 应用
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别名
ADRb1；ADRB1R；B1AR；Beta-1 adrenergic receptor
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P08588
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表达区间
1-477aa
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氨基酸序列
MGAGVLVLGASEPGNLSSAAPLPDGAATAARLLVPASPPASLLPPASESPEPLSQQWTAGMGLLMALIVLLIVAGNVLVIVAIAKTPRLQTLTNLFIMSLASADLVMGLLVVPFGATIVVWGRWEYGSFFCELWTSVDVLCVTASIETLCVIALDRYLAITSPFRYQSLLTRARARGLVCTVWAISALVSFLPILMHWWRAESDEARRCYNDPKCCDFVTNRAYAIASSVVSFYVPLCIMAFVYLRVFREAQKQVKKIDSCERRFLGGPARPPSPSPSPVPAPAPPPGPPRPAAAAATAPLANGRAGKRRPSRLVALREQKALKTLGIIMGVFTLCWLPFFLANVVKAFHRELVPDRLFVFFNWLGYANSAFNPIIYCRSPDFRKAFQRLLCCARRAARRRHATHGDRPRASGCLARPGPPPSPGAASDDDDDDVVGATPPARLLEPWAGCNGGAAADSDSSLDEPCRPGFASESKV
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分子量
52.6 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
The ADRB1 (adrenergic receptor beta 1) protein is a key player in the adrenergic signaling pathway, primarily involved in the regulation of cardiovascular function, including heart rate and contractility. It belongs to the G protein-coupled receptor (GPCR) family and is widely studied due to its significant role in pharmacology and medicine, particularly concerning heart diseases, hypertension, and heart failure. The interaction of ADRB1 with catecholamines, such as adrenaline and norepinephrine, triggers various intracellular signaling cascades that ultimately influence cardiac output and vascular tone. Research on recombinant ADRB1 protein has gained traction as it allows for the detailed study of receptor structure, function, and pharmacology in a controlled environment. Advances in recombinant DNA technology enable the production of large quantities of purified ADRB1 protein, facilitating its use in binding assays, pharmacological screening, and structural studies using techniques like X-ray crystallography and cryo-electron microscopy. Understanding the detailed mechanisms of ADRB1 signaling can lead to the development of new therapeutic strategies and drugs aimed at modulating its activity for various clinical conditions. The refinement of recombinant expression systems also paves the way for novel studies on receptor mutations and their implications in disease, further underscoring ADRB1's relevance in biomedical research.
