关键信息
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基因名
HSD17b3
- 应用
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别名
HSD17b3；EDH17B3；SDR12C2；17-beta-hydroxysteroid dehydrogenase type 3
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P37058
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表达区间
1-310aa
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氨基酸序列
MGDVLEQFFI LTGLLVCLAC LAKCVRFSRC VLLNYWKVLP KSFLRSMGQW AVITGAGDGI GKAYSFELAK RGLNVVLISR TLEKLEAIAT EIERTTGRSV KIIQADFTKD DIYEHIKEKL AGLEIGILVN NVGMLPNLLP SHFLNAPDEI QSLIHCNITS VVKMTQLILK HMESRQKGLI LNISSGIALF PWPLYSMYSA SKAFVCAFSK ALQEEYKAKE VIIQVLTPYA VSTAMTKYLN TNVITKTADE FVKESLNYVT IGGETCGCLA HEILAGFLSL IPAWAFYSGA FQRLLLTHYV AYLKLNTKVR
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分子量
34.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
HSD17B3, or 17beta-hydroxysteroid dehydrogenase type 3, is an enzyme that plays a critical role in steroid hormone biosynthesis, particularly in the conversion of androstenedione to testosterone, which is essential for male sexual differentiation and reproduction. Mutations in the HSD17B3 gene can lead to disorders of sexual development (DSDs) in males, characterized by underdeveloped genitalia and other reproductive anomalies. The study of recombinant HSD17B3 proteins has gained significance in understanding these pathological conditions and developing potential therapeutic strategies. By producing and purifying recombinant HSD17B3, researchers can investigate the enzyme's structure-function relationship, its catalytic mechanisms, and how specific mutations affect its activity. This research not only contributes to a deeper understanding of steroidogenesis but also aids in the identification of biomarkers for DSDs and informs clinical approaches for diagnosis and treatment. Additionally, recombinant HSD17B3 proteins hold promise for drug development that targets hormonal imbalances or provides insights into novel contraceptive methods. Understanding the function and regulation of HSD17B3 through recombinant studies is crucial for advancing both basic and clinical endocrinology.
