关键信息
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基因名
OPN1LW
- 应用
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别名
OPN1LW；RCP；Long-wave-sensitive opsin 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P04000
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表达区间
1-364aa
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氨基酸序列
MAQQWSLQRLAGRHPQDSYEDSTQSSIFTYTNSNSTRGPFEGPNYHIAPRWVYHLTSVWMIFVVTASVFTNGLVLAATMKFKKLRHPLNWILVNLAVADLAETVIASTISIVNQVSGYFVLGHPMCVLEGYTVSLCGITGLWSLAIISWERWLVVCKPFGNVRFDAKLAIVGIAFSWIWSAVWTAPPIFGWSRYWPHGLKTSCGPDVFSGSSYPGVQSYMIVLMVTCCIIPLAIIMLCYLQVWLAIRAVAKQQKESESTQKAEKEVTRMVVVMIFAYCVCWGPYTFFACFAAANPGYAFHPLMAALPAYFAKSATIYNPVIYVFMNRQFRNCILQLFGKKVDDGSELSSASKTEVSSVSSVSPA
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分子量
43.4 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
OPN1LW, a member of the opsin family, is a crucial protein involved in the phototransduction process in vertebrates, particularly in the functioning of long-wavelength (red) sensitive photoreceptor cells in the retina. Research on OPN1LW has gained significant attention due to its unique role in human color vision, especially in distinguishing between red and green wavelengths. Mutations and variations in the OPN1LW gene are associated with color vision deficiencies, such as protanopia and protanomaly, highlighting its importance in visual perception. Additionally, OPN1LW is of interest in the study of evolutionary biology, as it provides insights into how color vision has adapted in different species. The characterization and recombinant expression of OPN1LW protein have become pivotal in understanding its structure-function relationships and developing potential therapies for vision-related disorders. Researchers employ various techniques, including molecular cloning, expression in suitable systems, and functional assays, to investigate the protein's properties. Such studies aim to elucidate the mechanisms underlying color vision and may pave the way for advancements in gene therapy, allowing for the correction of inherited visual defects linked to OPN1LW dysregulation. Overall, OPN1LW research bridges fundamental science with potential clinical applications, making it a focus area in both genetics and ophthalmology.
