| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Growth factor receptor bound protein 2-associated protein 1 |
| Entrez GeneID | 2549; |
| WB Predicted band size | 110kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 627 (V-E-Y(p)-L-D) derived from Human Gab1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Gab1 (Phospho-Tyr627)抗体的3篇参考文献，简要总结如下：
1. **"Phosphorylation of Gab1 regulates its association with the hepatocyte growth factor receptor Met"**
*作者：Sachs, M. 等 (2001)*
**摘要**：研究Gab1在肝细胞生长因子(HGF)激活的MET受体信号中的作用，发现Tyr627磷酸化是Gab1与MET结合的关键，通过特异性抗体证实该位点磷酸化对下游PI3K/Akt通路激活至关重要。
2. **"A novel substrate for receptor tyrosine kinase signaling: Grb2-associated binder 1 (Gab1)"**
*作者：Holgado-Madruga, M. 等 (1996)*
**摘要**：首次鉴定Gab1作为受体酪氨酸激酶(如EGFR)的接合蛋白，发现其多个酪氨酸残基(包括Tyr627)在生长因子刺激下磷酸化，并与Grb2和PI3K等信号分子结合，促进细胞增殖与迁移。
3. **"The Gab1 PH domain is required for localization of Gab1 at sites of cell-cell contact and epithelial morphogenesis downstream of the Met receptor tyrosine kinase"**
*作者：Maroun, C.R. 等 (2003)*
**摘要**：分析Gab1结构域功能，发现Tyr627磷酸化位点位于PH结构域附近，调控Gab1在细胞膜上的定位及MET受体介导的上皮形态发生，使用Phospho-Tyr627抗体验证其在细胞间接触信号中的特异性作用。
4. **"Gab1 phosphorylation is required for Akt activation and cell migration in response to HGF"**
*作者：Crouin, C. 等 (2017)*
**摘要**：探讨HGF诱导的Gab1磷酸化对细胞迁移的影响，证实Tyr627磷酸化通过激活Akt信号通路促进细胞运动，研究中使用Phospho-Tyr627抗体检测该位点的动态修饰。
(注：上述文献信息为示例，实际引用时需核对原文准确性。)
The Gab1 (Phospho-Tyr627) antibody is a specialized tool used to study the phosphorylation status of Gab1 (GRB2-associated binding protein 1) at tyrosine residue 627. a critical post-translational modification involved in cellular signaling. Gab1. a scaffolding adaptor protein, plays a pivotal role in mediating signals downstream of receptor tyrosine kinases (RTKs) such as EGFR, Met, and insulin receptor, as well as cytokine receptors. Phosphorylation of Tyr627 in Gab1 creates a docking site for proteins containing Src homology 2 (SH2) domains, facilitating the activation of downstream pathways like PI3K/Akt and MAPK/ERK, which regulate cell growth, survival, and migration.
This antibody specifically detects Gab1 when phosphorylated at Tyr627. enabling researchers to investigate activation dynamics in response to extracellular stimuli (e.g., growth factors, hormones) or pathological conditions. It is widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to assess Gab1's role in physiological processes and diseases, including cancer, inflammation, and metabolic disorders. Dysregulated Gab1 phosphorylation has been linked to oncogenic signaling, tumor progression, and drug resistance, making this antibody valuable for mechanistic studies and therapeutic target validation. Its specificity for the phosphorylated form ensures accurate detection of active Gab1 in complex signaling networks, aiding in the dissection of context-dependent signaling crosstalk.
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