| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Inward rectifier potassium channel 13, Inward rectifier K(+) channel Kir71, Potassium channel, inwardly rectifying subfamily J member 13, KCNJ13 |
| Entrez GeneID | 3769 |
| WB Predicted band size | 40.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This KCNJ13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 67-95 amino acids from the N-terminal region of human KCNJ13. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于KCNJ13 (N-term)抗体的3篇参考文献，信息基于公开研究整理：
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1. **文献名称**：*KCNJ13 mutations associated with Leber congenital amaurosis cause impaired potassium channel function*
**作者**：Sergouniotis PI et al.
**摘要**：研究通过Western blot和免疫荧光技术，利用KCNJ13 (N-term)抗体证实了突变体Kir7.1蛋白在视网膜细胞中的表达异常，揭示了其与先天性黑蒙症相关的功能缺陷。
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2. **文献名称**：*Expression and localization of Kir7.1 in the murine retinal pigment epithelium*
**作者**：Kusaka S et al.
**摘要**：使用KCNJ13 (N-term)抗体进行免疫组化分析，发现Kir7.1通道蛋白在小鼠视网膜色素上皮细胞极性分布中的关键作用，提示其参与离子稳态调控。
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3. **文献名称**：*Functional characterization of KCNJ13 variants linked to inherited retinal dystrophy*
**作者**：Patel N et al.
**摘要**：通过免疫印迹和共聚焦显微镜，结合KCNJ13 (N-term)抗体，验证了致病性突变导致Kir7.1蛋白表达减少及细胞内运输障碍，为基因治疗提供依据。
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**注**：若需具体文献来源，建议通过PubMed或Google Scholar以关键词“KCNJ13 antibody N-terminal”进一步检索。部分研究可能使用商业化抗体(如Sigma-Aldrich的HPA编号)，可参考供应商提供的应用文献。
The KCNJ13 (N-term) antibody is a targeted reagent used to detect the N-terminal region of the potassium inwardly rectifying channel subfamily J member 13 (KCNJ13) protein. KCNJ13 encodes Kir7.1. a member of the inward rectifier potassium (Kir) channel family, which plays critical roles in regulating cellular membrane potential and potassium ion homeostasis. Kir7.1 is primarily expressed in retinal pigment epithelium (RPE), the gastrointestinal tract, and certain brain regions, where it contributes to transepithelial transport, neuronal signaling, and ion balance. Mutations in KCNJ13 are linked to retinal disorders, including Leber congenital amaurosis and snowflake vitreoretinal degeneration, highlighting its importance in visual function.
The N-terminal-specific antibody is designed to recognize epitopes within the initial segment of Kir7.1. enabling researchers to study its expression, localization, and interactions in tissues or cell models. It is commonly employed in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate KCNJ13's role in physiological and pathological contexts. Validation of this antibody typically involves testing in overexpression systems (e.g., HEK293 cells) and knockout controls to confirm specificity. Its applications extend to exploring disease mechanisms, particularly retinal degeneration, and evaluating therapeutic strategies targeting Kir7.1 dysfunction. By enabling precise detection, this antibody serves as a vital tool for advancing understanding of KCNJ13's contributions to cellular physiology and human disease.
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