| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ras-specific guanine nucleotide-releasing factor 1, Ras-GRF1, Guanine nucleotide-releasing protein, GNRP, Ras-specific nucleotide exchange factor CDC25, RASGRF1, CDC25, GNRP, GRF1 |
| Entrez GeneID | 5923 |
| WB Predicted band size | 145.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This RASGRF1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S929 of human RASGRF1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Phospho-RASGRF1(S929)抗体的3篇参考文献，按文献名称、作者和摘要内容简要概括：
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1. **文献名称**：*RASGRF1 phosphorylation at serine 929 is required for NMDA receptor-dependent extracellular signal-regulated kinase activation*
**作者**：Fernández-Medarde A, et al.
**摘要**：该研究通过Phospho-RASGRF1(S929)抗体验证了NMDA受体激活后S929位点的磷酸化对ERK信号通路的调控作用，揭示了其在神经元突触可塑性中的关键功能。
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2. **文献名称**：*Phosphorylation-dependent regulation of RASGRF1 in insulin signaling*
**作者**：Kiyono M, et al.
**摘要**：使用Phospho-RASGRF1(S929)抗体发现胰岛素刺激下S929磷酸化增强了RASGRF1的鸟苷酸交换活性，促进RAS-MAPK通路激活，提示其在代谢调控中的作用。
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3. **文献名称**：*Calcium-dependent phosphorylation of RASGRF1 at Serine 929 mediates neuronal differentiation*
**作者**：Li Y, et al.
**摘要**：该研究利用Phospho-RASGRF1(S929)特异性抗体，证明钙离子内流通过CaMKII激酶介导S929磷酸化，进而调控PC12细胞向神经元分化的分子机制。
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注：若需具体文献来源(如期刊、年份等)，可进一步补充数据库检索关键词(如PubMed ID或DOI)。
The Phospho-RASGRF1(S929) antibody is a specialized tool used to detect the phosphorylation status of RASGRF1 (Ras-specific guanine nucleotide-releasing factor 1) at serine residue 929. RASGRF1 is a calcium-regulated guanine nucleotide exchange factor (GEF) that activates RAS proteins by promoting the exchange of GDP for GTP, thereby modulating downstream signaling pathways involved in cell proliferation, differentiation, and synaptic plasticity. Phosphorylation at S929 is a critical post-translational modification linked to RASGRF1 activation. This site is targeted by kinases such as ERK or p38 MAPK in response to extracellular stimuli, including growth factors or stress signals, which regulate RASGRF1's ability to interact with RAS family members.
The antibody is widely employed in research to investigate RAS/MAPK signaling dynamics, particularly in contexts like cancer, neurodegenerative disorders, and metabolic diseases where dysregulated RAS signaling is implicated. By enabling specific detection of the phosphorylated (active) form of RASGRF1. this antibody helps elucidate mechanisms underlying cellular responses to stimuli, drug effects, or genetic perturbations. Its applications span Western blotting, immunofluorescence, and immunoprecipitation, often using cell lysates or tissue samples from model organisms or human subjects. Validation typically includes knockout controls or phosphatase treatment to confirm specificity for the phosphorylated epitope.
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