Rabbit Polyclonal MYO6(C-termR1181) Antibody

The MYO6 (C-term R1181) antibody is a specialized tool for detecting myosin VI (MYO6), a motor protein critical for intracellular vesicle trafficking, organelle positioning, and maintenance of stereocilia in hair cells of the inner ear. MYO6 is unique among myosins as it moves toward the minus end of actin filaments, enabling roles in endocytosis, autophagy, and cell migration. The antibody specifically targets the C-terminal region of MYO6. recognizing an epitope around residue arginine 1181 (R1181). This region is functionally significant, as mutations in MYO6’s C-terminal domain are linked to hereditary hearing loss (DFNA22. DFNB37) and hypertrophic cardiomyopathy. The R1181 epitope lies near binding sites for adaptor proteins (e.g., Dab2. TOM1) and the PDZ domain-interacting motif, which mediates interactions critical for MYO6’s role in clathrin-mediated endocytosis and Golgi morphology. Researchers use this antibody in Western blotting, immunofluorescence, and immunohistochemistry to study MYO6 expression, localization, and dysfunction in tissues like cochlea, kidney, and cardiac muscle. Its specificity for the C-terminus helps distinguish full-length MYO6 from truncated isoforms. Validated in human and mouse models, the antibody aids in exploring MYO6-related pathologies, including progressive hearing loss and cardiac hypertrophy, as well as its broader functions in cellular trafficking and polarity.