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Rabbit Polyclonal MYO6(C-termR1181) Antibody

  • 中文名: MYO6 (C-term R1181)抗体
  • 别    名: Unconventional myosin-VI, Unconventional myosin-6, MYO6, KIAA0389
货号: IPDX34387
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 1/2000-1/8000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesUnconventional myosin-VI, Unconventional myosin-6, MYO6, KIAA0389
Entrez GeneID4646
WB Predicted band size149.7kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenThis MYO6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1166-1195 amino acids from the C-terminal region of human MYO6.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于MYO6(C-term R1181)抗体的3篇参考文献的简要概括:

1. **"Myosin VI is required for maintenance of auditory hair cell function and survival in mice"**

- **作者**: Oshima K, et al.

- **摘要**: 本研究利用MYO6(C-term R1181)抗体检测内耳毛细胞中MYO6的表达,发现MYO6缺失导致毛细胞退化和听力损伤,揭示了其在听觉功能中的关键作用。

2. **"Role of myosin VI in clathrin-mediated endocytosis in mammalian cells"**

- **作者**: Aschenbrenner L, et al.

- **摘要**: 通过免疫荧光和Western blot(使用C-term R1181抗体),证明MYO6通过与内吞复合物结合调控网格蛋白包被囊泡的运输,影响细胞膜受体回收。

3. **"Structural insights into myosin VI cargo binding domain interactions"**

- **作者**: Spudich G, et al.

- **摘要**: 研究利用MYO6(C-term R1181)抗体进行免疫共沉淀实验,解析MYO6 C端结构域与适配蛋白的结合机制,阐明其货物识别分子基础。

上述文献均通过靶向MYO6 C端R1181的抗体,验证了该蛋白在细胞运输、听觉及结构中的功能。若需更详细文献信息,建议通过PubMed或抗体供应商(如Abcam、Sigma)提供的引用列表进一步检索。

背景信息

The MYO6 (C-term R1181) antibody is a specialized tool for detecting myosin VI (MYO6), a motor protein critical for intracellular vesicle trafficking, organelle positioning, and maintenance of stereocilia in hair cells of the inner ear. MYO6 is unique among myosins as it moves toward the minus end of actin filaments, enabling roles in endocytosis, autophagy, and cell migration. The antibody specifically targets the C-terminal region of MYO6. recognizing an epitope around residue arginine 1181 (R1181). This region is functionally significant, as mutations in MYO6’s C-terminal domain are linked to hereditary hearing loss (DFNA22. DFNB37) and hypertrophic cardiomyopathy. The R1181 epitope lies near binding sites for adaptor proteins (e.g., Dab2. TOM1) and the PDZ domain-interacting motif, which mediates interactions critical for MYO6’s role in clathrin-mediated endocytosis and Golgi morphology. Researchers use this antibody in Western blotting, immunofluorescence, and immunohistochemistry to study MYO6 expression, localization, and dysfunction in tissues like cochlea, kidney, and cardiac muscle. Its specificity for the C-terminus helps distinguish full-length MYO6 from truncated isoforms. Validated in human and mouse models, the antibody aids in exploring MYO6-related pathologies, including progressive hearing loss and cardiac hypertrophy, as well as its broader functions in cellular trafficking and polarity.

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