Rabbit Polyclonal DNMT3L Antibody

DNMT3L (DNA methyltransferase 3-like) is a regulatory protein critical for establishing genomic DNA methylation patterns during mammalian development. Unlike catalytically active DNMT3A and DNMT3B, DNMT3L lacks intrinsic methyltransferase activity but serves as a cofactor by stabilizing and enhancing the enzymatic function of DNMT3A/3B. It plays a vital role in germ cell development, genomic imprinting, and X-chromosome inactivation by facilitating de novo DNA methylation at specific loci, particularly in oocytes and primordial germ cells. Antibodies targeting DNMT3L are essential tools for studying its expression, localization, and interaction partners in developmental and disease contexts. These antibodies are widely used in techniques like Western blotting, immunofluorescence, chromatin immunoprecipitation (ChIP), and immunohistochemistry to explore DNMT3L's role in epigenetic regulation. Researchers also employ DNMT3L antibodies to investigate its dysregulation in cancers, imprinting disorders (e.g., Prader-Willi syndrome), and infertility. Most commercial DNMT3L antibodies are raised against conserved regions of the human or mouse protein, with validation typically including knockout cell lines or tissues to confirm specificity. As aberrant DNA methylation is linked to numerous diseases, DNMT3L antibodies remain crucial for advancing our understanding of epigenetic mechanisms and their therapeutic targeting.