| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Protein boule-like, BOLL, BOULE |
| Entrez GeneID | 66037 |
| WB Predicted band size | 31.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This BOLL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-51 amino acids from the N-terminal region of human BOLL. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与BOLL(N-term)抗体相关的参考文献及其摘要概括：
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1. **文献名称**: *Boule is essential for spermatogenesis in zebrafish*
**作者**: Xu, H.Y. et al.
**摘要**: 研究利用斑马鱼模型，通过N端特异性抗体检测Boule蛋白在生殖细胞中的表达，证实其在减数分裂启动和精子形成中的关键作用，抗体特异性通过敲除实验验证。
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2. **文献名称**: *A conserved germ cell-specific antibody recognizes Boule in diverse species*
**作者**: Li, M. & Matzuk, M.M.
**摘要**: 报道了一种抗BOLL N端表位的多克隆抗体的开发，该抗体在小鼠、果蝇和人类生殖细胞中均能特异性标记Boule蛋白，支持其在进化中的功能保守性研究。
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3. **文献名称**: *BOLL gene disruption causes spermatogenic failure in humans and mice*
**作者**: Zhang, Y. et al.
**摘要**: 通过免疫组化(使用N-term抗体)和基因编辑技术，揭示BOLL缺失导致精子发生阻滞，抗体定位显示蛋白在减数分裂前生殖细胞核内高表达。
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注：以上文献为示例，实际文献需通过PubMed或专业数据库检索确认。如需具体文章，可提供更详细的研究方向或应用场景以便精准推荐。
The BOLL (Boule homolog) protein, encoded by the *BOLL* gene, is a conserved RNA-binding protein critical for germ cell development, particularly in spermatogenesis. As a member of the DAZ (Deleted in Azoospermia) family, it shares structural features like RNA recognition motifs (RRMs) and plays roles in regulating mRNA stability, splicing, and translation during meiosis. BOLL is predominantly expressed in germ cells, with highest levels observed in the testes, where it ensures proper progression of male germ cell maturation. Dysregulation of BOLL has been linked to male infertility and germ cell tumors, such as seminomas and ovarian dysgerminomas.
The BOLL (N-term) antibody is a specific tool designed to target the N-terminal region of the BOLL protein. This region often contains conserved domains essential for protein-protein or protein-RNA interactions. Researchers use this antibody in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study BOLL's expression patterns, subcellular localization (typically cytoplasmic), and functional dynamics in reproductive tissues or cancer models. Its specificity makes it valuable for exploring BOLL's role in fertility disorders, tumorigenesis, and as a potential biomarker for germ cell malignancies. Studies also leverage it to differentiate BOLL from related DAZ family members (e.g., DAZL, BOULE) due to its N-terminal epitope focus.
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