Rabbit Polyclonal NANOG(S285) Antibody

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     All lanes : Anti-NANOG Antibody (S285) at 1:1000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: human testis lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 35 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Western blot analysis of NANOG Antibody (S285) (Cat.# AP1486e) in HepG2 cell line lysates (35ug/lane). NANOG (arrow) was detected using the purified Pab.    

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     Fluorescent confocal image of SY5Y cells stained with AP1486e NANOG (S285) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with AP1486e NANOG (S285) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (5.25 μM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 3 min). Nanog immunoreactivity is localized mainly to the nuclei and also to the cytoplasm.    

NANOG (S285) antibody is a specialized tool used to detect the NANOG protein, a key transcription factor critical for maintaining pluripotency in embryonic stem cells (ESCs) and primordial germ cells. Named after the Celtic mythological land of eternal youth "Tír na nÓg," NANOG plays a pivotal role in self-renewal and cell fate determination by suppressing differentiation signals. The antibody specifically recognizes NANOG phosphorylated at serine 285 (S285), a post-translational modification linked to protein stability, transcriptional activity, and interactions with other pluripotency regulators like OCT4 and SOX2.

This rabbit monoclonal antibody (clone number often specified) is widely used in stem cell research, developmental biology, and cancer studies, as aberrant NANOG expression is associated with tumorigenesis and cancer stem cell properties. Applications include Western blotting, immunofluorescence, immunoprecipitation, and chromatin immunoprecipitation (ChIP), typically validated in human and mouse samples. Researchers rely on its specificity to explore mechanisms of pluripotency, cellular reprogramming (e.g., induced pluripotent stem cells), and NANOG's role in diseases. Quality control often involves testing in knockout models or siRNA-treated cells to confirm signal depletion. Proper storage (e.g., -20°C with carrier proteins) and optimized dilution protocols are essential for reproducibility.