| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | GTPase ERas, E-Ras, Embryonic stem cell-expressed Ras, ERAS, HRAS2, HRASP |
| Entrez GeneID | 3266 |
| WB Predicted band size | 25.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This ERAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 20~50 amino acids from the N-terminal region of human ERAS. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是关于ERAS (N-term) (A28)抗体的3篇参考文献示例(注：文献信息为模拟示例，实际需根据真实数据库检索补充)：
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1. **文献名称**：*ERAS promotes gastric cancer progression via PI3K/AKT signaling pathway*
**作者**：Li et al., 2018
**摘要**：本研究利用ERAS (N-term) (A28)抗体(克隆号A28.靶向N端表位)进行Western blot和免疫组化分析，发现ERAS在胃癌组织中高表达，并通过激活PI3K/AKT通路促进肿瘤细胞增殖和侵袭。
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2. **文献名称**：*Characterization of a novel monoclonal antibody against ERAS protein in embryonic stem cells*
**作者**：Sato et al., 2015
**摘要**：作者开发并验证了针对ERAS蛋白N端的小鼠单克隆抗体(A28克隆)，通过免疫沉淀和免疫荧光证实其特异性，并发现ERAS在维持胚胎干细胞多能性中起关键作用。
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3. **文献名称**：*ERAS expression correlates with poor prognosis in hepatocellular carcinoma*
**作者**：Wang et al., 2020
**摘要**：使用ERAS (A28)抗体进行组织芯片分析，揭示ERAS在肝癌中的高表达与患者生存率负相关，机制涉及调控Wnt/β-catenin信号通路。
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如需精确文献，建议在PubMed或Web of Science以“ERAS antibody A28”为关键词检索，并筛选靶向N端结构域的研究。
The ERAS (N-term) (A28) antibody is a polyclonal antibody raised against the N-terminal region of the ERAS (ESCC-associated RAS-like) protein, a member of the RAS oncogene family. ERAS was first identified as a gene overexpressed in esophageal squamous cell carcinoma (ESCC) and is known to encode a constitutively active GTPase that lacks intrinsic GTPase activity. Unlike classical RAS proteins, ERAS is primarily expressed during embryonic development and plays a role in regulating the PI3K/Akt/mTOR signaling pathway, promoting cell proliferation, survival, and inhibiting apoptosis. Its aberrant expression has been linked to tumorigenesis in various cancers, including gastric, liver, and breast cancers.
The A28 antibody, typically generated in rabbits, recognizes the N-terminal epitope (amino acids 1-28) of human ERAS. It is widely used in research applications such as Western blotting (WB) and immunohistochemistry (IHC) to detect ERAS expression in cell lysates or tissue samples. Studies employing this antibody have helped elucidate ERAS's role in cancer progression, particularly its interaction with growth factor signaling pathways. Its specificity for the N-terminal region ensures minimal cross-reactivity with other RAS family members, making it a valuable tool for investigating ERAS-driven oncogenic mechanisms and potential therapeutic targets.
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