Rabbit Polyclonal TOLLIP Antibody

The TOLLIP (Toll-interacting protein) antibody is a crucial tool in studying innate immune responses and cellular signaling pathways. TOLLIP, a ubiquitin-binding protein, acts as a negative regulator of Toll-like receptor (TLR) signaling by modulating interleukin-1 receptor (IL-1R) and TLR-mediated inflammatory responses. It interacts with key components like IRAK1 and TRAF6. facilitating ubiquitination processes and suppressing excessive immune activation. Researchers use TOLLIP antibodies primarily in Western blotting, immunofluorescence, and immunoprecipitation to detect TOLLIP expression, localization, and protein-protein interactions in various cell types, including immune cells and cancer models. These antibodies help elucidate TOLLIP's dual role in autophagy and endosomal sorting, particularly its involvement in phosphatidylinositol 3-phosphate binding and vesicle trafficking. Studies leveraging TOLLIP antibodies have linked its dysregulation to chronic inflammatory diseases, infections, and cancer progression, making it a potential therapeutic target. Commercial TOLLIP antibodies are typically developed in hosts like rabbits or mice, targeting specific epitopes within its N-terminal or C-terminal domains. Validation includes knockout cell line controls to ensure specificity, given TOLLIP's low molecular weight (~~22 kDa) and structural similarities to other ubiquitin-related proteins.