| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Protein Wnt-8b, WNT8B |
| Entrez GeneID | 7479 |
| WB Predicted band size | 38.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This WNT8B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 323-351 amino acids from the C-terminal region of human WNT8B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于WNT8B抗体的参考文献示例(注：以下内容为模拟示例，非真实文献)：
1. **文献名称**：*WNT8B regulates neural crest migration in avian embryos*
**作者**：Thompson RL, et al.
**摘要**：本研究利用WNT8B特异性抗体，通过免疫组织化学技术揭示了WNT8B在鸡胚胎神经嵴细胞迁移中的动态表达模式，证明其通过调控β-catenin信号通路影响细胞运动。
2. **文献名称**：*Aberrant WNT8B expression in colorectal cancer and its clinical implications*
**作者**：Chen H, et al.
**摘要**：通过WNT8B抗体对结直肠癌组织样本进行检测，发现WNT8B在肿瘤边缘高表达，并与患者淋巴结转移和预后不良显著相关，提示其作为潜在生物标志物的价值。
3. **文献名称**：*WNT8B antibody-mediated inhibition disrupts early brain patterning in Xenopus*
**作者**：Garcia M, et al.
**摘要**：在非洲爪蟾胚胎中，使用中和性WNT8B抗体阻断其信号传导，导致前脑和中脑发育异常，证实WNT8B在脊椎动物头部区域形成中的关键作用。
4. **文献名称**：*Characterization of a novel monoclonal antibody against human WNT8B for functional studies*
**作者**：Kimura T, et al.
**摘要**：开发并验证了一种高亲和力单克隆WNT8B抗体，通过体外实验证实其可特异性抑制WNT/β-catenin通路活性，为靶向WNT8B的疾病治疗提供工具。
(注：以上文献为示例性质，实际引用请通过学术数据库检索真实文献。)
The Wnt8b antibody is a specialized tool used to detect and study the Wnt8b protein, a member of the Wnt family of secreted signaling molecules. Wnt proteins play critical roles in embryonic development, tissue homeostasis, and stem cell regulation by activating canonical (β-catenin-dependent) and non-canonical signaling pathways. Specifically, Wnt8b is implicated in early embryogenesis, neural patterning, and organogenesis, particularly in the development of the brain, eyes, and limbs. Dysregulation of Wnt8b has been linked to developmental disorders and cancers, making it a target for research in developmental biology and oncology.
Antibodies against Wnt8b are typically generated in hosts like rabbits or mice using immunogenic peptides or recombinant proteins corresponding to conserved regions of the Wnt8b sequence. These antibodies are validated for applications such as Western blotting (WB), immunohistochemistry (IHC), immunofluorescence (IF), and enzyme-linked immunosorbent assays (ELISA). Researchers rely on Wnt8b antibodies to investigate its expression patterns, subcellular localization, and interactions with other signaling components. Specificity is confirmed through knockout controls or siRNA-mediated knockdown to ensure minimal cross-reactivity with related Wnt family members (e.g., Wnt8a). Commercial Wnt8b antibodies often include validation data from peer-reviewed studies, enhancing their reliability. Current research focuses on Wnt8b's role in neural crest cell migration, retinal development, and its potential as a biomarker or therapeutic target in diseases like colorectal cancer or neurodegenerative conditions.
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